TABLE 2.

Characteristics of ORFs and deduced amino acid sequences present in the sequenced DNA fragment

ORFGeneProduct size (no. of amino acids)ORF location (nt)aProtein (description) to which ORF product exhibits homologySourceIdentitybAccession no.
%Range (aa)
1tnpA1363-413TnpA; transposase of Tn1721, truncatedE. coli95135P51565
2ycjA107791-468YcjA; putative bacterial repressor proteinPlasmid R64; S. enterica serovar Typhimurium100107BAB91595
3ydaA377896-2029YdaA; putative permeasePlasmid R64; S. enterica serovar Typhimurium90377BAB91596
4ydbA2052939-2322YdbAPlasmid R64; S. enterica serovar Typhimurium96205BAB91597
5ibfA5184686-3130lbfA; putative ABC transporterPlasmid R64; S. enterica serovar Typhimurium95518BAB91598
6ydeA1965539-4949YdeAPlasmid R64; S. enterica serovar Typhimurium100196BAB91599
7ydfA855796-5539YdfA; putative ABC transporterPlasmid R64; S. enterica serovar Typhimurium10085BAB91600
8ydfB7126150-8288YdfB; putative ABC transporterPlasmid R64; S. enterica serovar Typhimurium100712BAB91601
9mck1338851-8450Mck; involved in coordination of plasmid replication with cell divisionPlasmid R64; S. enterica serovar Typhimurium99133BAB91602
10kor769093-8863Kor; involved in coordination of plasmid replication with cell divisionPlasmid R64; S. enterica serovar Typhimurium10076BAB91603
11ydiA969389-9679YdiA; 93% identical to protein YebA on E. coli plasmid FPlasmid R64; S. enterica serovar Typhimurium10096BAB91604
12ydiA2999669-10568YdjA; 100% identical to protein YebB on E. coli plasmid FPlasmid R64; S. enterica serovar Typhimurium100299BAB91605
13pifA*44311949-10618Truncated ORF; homologous to residues 299 to 714 of 741 residues of protein PifA; phage T7 exclusion proteinPlasmid R64; S. enterica serovar Typhimurium100443BAB91606
P96329
14tnpA22412299-12973TnpA; transposase of IS903Insertion sequence IS90399223AAF30382
15iroN72513385-15562IroN; enterochelin and dihydrobenzoic acid receptorPAI III of E. coli strain 53699725CAC43424
16iroE31816563-15607IroEPAI III of E. coli strain 53699318CAC43425
17iroD40917877-16648IroD; putative ferric enterochelin esterasePAI III of E. coli strain 53699409CAC43426
18iroC1,26121766-17981IroC; putative ABC transporterPAI III of E. coli strain 536991,261CAC43427.2
19iroB37122895-21780IroB; putative UDP-glucoronosyl and UDP-glucosyl transferasePAI III of E. coli strain 536100371CAD66179.1
208923363-23094Hypothetical proteinNo significant homology
2117324692-24171Hypothetical protein; putative integraseE. coli strain K559100CAA54707
229625164-24874Hypothetical proteinE. coli strain K55948CAA54710
239726026-25733Hypothetical proteinE. coli8076AAB40752
24iss10226347-26039Iss (increased serum survival and complement resistance)E. coli strain 102100102AAD41540
258026948-26706Hypothetical proteinE. coli strain EDL93310026AE005326
2611226959-27297Putative transposase of insertion element IS2E. coli98109P51026
2726327404-28195Putative integrase of insertion element IS2E. coli97262P51026
285428530-28366Hypothetical protein; homologous to gene trp1328 on IS1328Yersinia enterocolitica6350CAB46575
2939729418-30611Hypothetical protein; putative cobalamin synthesis proteinE. coli strain CFT07365397NP_753181
3010330750-31061Hypothetical protein; putative integraseShigella flexneri 2a strain 3015982NP_709264
3120731248-31869Truncated ORF; homologous to gene HCM1.201 on plasmid pHCM1; putative transposaseS. enterica serovar Typhi strain CT1895207NP_569402
  • a Nucleotide positions from the start to stop codons in the pJS332 sequence.

  • b Identity is presented as percentage of amino acid identity between the pJS332 ORF and the best hit as determined with BLAST and FASTA. The range is the number of amino acids (aa) over which this identity exists.