TABLE 1.

Codon usage in native PA83, E. coli codon-optimized PA83ec, and S. enterica serovar Typhimuriuma

Amino acidCodonFrequency/1,000 codons in:
PA83PA83ecSalmonella
RCGA2.70.03.5
CGC0.010.822.5
CGG5.40.06.2
CGU4.127.119.9
AGA21.70.03.1
AGG4.10.02.0
LCUA4.10.04.6
CUC2.71.39.9
CUG1.458.449.4
CUU8.214.910.5
UUA51.610.813.4
UUG9.52.711.7
SUCA19.00.07.4
UCC4.125.810.9
UCG9.50.08.3
UCU28.547.58.7
AGC1.413.516.4
AGU31.26.77.6
TACA28.51.36.8
ACC8.244.824.1
ACG13.68.115.5
ACU25.821.78.3
PCCA13.68.16.5
CCC4.11.36.0
CCG8.224.423.7
CCU12.22.77.7
AGCA23.113.513.5
GCC2.78.126.7
GCG9.510.837.2
GCU17.721.714.7
GGGA25.80.07.4
GGC2.721.734.3
GGG14.90.011.3
GGU4.124.419.6
VGUA23.114.912.2
GUC2.76.717.7
GUG13.610.824.8
GUU14.921.715.8
KAAA59.847.536.7
AAG17.129.812.0
NAAC21.774.721.3
AAU70.717.620.2
QCAA35.312.212.8
CAG5.428.531.0
HCAC1.48.19.4
CAU12.26.711.9
EGAA51.644.838.4
GAC8.236.621.7
DGAG16.323.021.5
GAU55.727.134.1
YUAC8.227.112.6
UAU29.910.817.6
CUGC0.00.06.3
UGU0.00.05.0
FUUC6.820.315.3
UUU25.812.222.5
IAUA21.70.06.7
AUC13.648.924.8
AUU38.023.028.2
MAUG10.910.925.5
WUGG9.59.511.8
  • a Data for PA83 codon usage were derived from (Williamson et al., (8 July 2001, British Patent Office). Data for S. enterica serovar Typhimurium codon usage were derived from Tzschaschel et al. (28).