TABLE 3.

Primers used in this study

PrimerSequence (5′-3′)
Cox-Bam-JB CGGGGGATCCAGCTAATTGGATAAACCGCGAG
Cox-Bam-PO CGGGGGATCCACGTCTGCTTCACGGAAAGGAC
Cox-Bam-Q CGGGGGATCCATTAGCGGTTTGATCCGTTGCG
Cox-Bam-TS CGGGGGATCCAGGGAATTTTCCTCATTATGCG
Cox-Bam-WX CGGGGGATCCGCTCCTTGAAAGTTGATGTCCG
Cox-Nde-JB CTAGCCGCATATGGCTTTGCGCAACATTCAAT
Cox-Nde-PO CTAGCCGCATATGTATCCAGCGCAACAAAACG
Cox-Nde-Q CTAGCCGCATATGACATCATTTGACTTGTCCAAC
Cox-Nde-TS CTAGCCGCATATGAAATCTCTCGATGAGGCGC
Cox-Nde-WX CTAGCCGCATATGCCAGATCTGTCGCATAAAG
Lpn-EI-J GCCGGAATTCGGCATTATTATTCCTTCCTTC
Lpn-EI-P GCCGGAATTCTATATCGATACTCCAATGGCC
Lpn-EI-Q GCCGGAATTCAGCCATGATGAACGTGGTTTC
Lpn-EI-T GCCGGAATTCGTTAGCTAAAATGCAAGGGAAC
Lpn-EI-W GCCGGAATTCTGATCCTGATTTCTTTTTCATATT
Lpn-Nde-JB CTAGCCGCATATGTTGGTTCACATTCAGTCAATTTTC
Lpn-Nde-PO CTAGCCGCATATGTATATACAATTAGTTAATAGATTC TATATA
Lpn-Nde-Q CTAGCCGCATATGTTCAATACTTATGGGAACCAAG
Lpn-Nde-TS CTAGCCGCATATGTAAACCTCAAAAAATCATCCTTTA
Lpn-Nde-WX CTAGCCGCATATGTTACTCTTTTTTTACTGAGATACG
Ls-F-T18 CCAACTCGAGAATGGAGCGAGATATTAGCAAG
Ls-F-T25 GGAACTGCAGGGATGGAGCGAGATATTAGCAAG
Ls-R-T18 GGAAAAGCTTATATCATACATTAACTCATCCAGGGG
Ls-R-T25 GGAAGGTACCCTAATCATACATTAACTCATCCAGGG
Lw-F-T18 CCTTCTCGAGAATGCCTGATTAAGCCATGAAGCC
Lw-F-T25 GGAACTGCAGGGATGCCTGATTTAAGCCATGAAGCC
Lw-R-T18 GGAAAAGCTTATTTCATCCCCTTCGAGTGCTCG
Lw-R-T25 GGAAGGTACCTTATTCATCCCCTTCGAGTGCTCG
J-Down-F GTCGACAGGGCAAAATTCAGGAAGC
J-Down-R CACATGTGAAGGAAGAATACGAG
J-Up-F GTTTCCTCCGTACTGCAAAC
J-Up-R GTCGACAGAATATAAACGCCAGGAACC
P-Down-F CCAGTCGACAGACGTTTATG
P-Down-R AGCGCTCGCATCAAGAAGAATTGCCC
P-Up-F AGCGCTGTTTGGTGCTGCTGAATCTG
P-Up-R GTCGACTATGACGATCCATACTGGCG
S-T18-F CCCTCGAGGATGCAACTTGCGAATAAATTAACGG
S-T18-R TCAAGCTTATATCGTACATCAGTTCATCCAGCG
S-T25-F GGCTGCAGGGATGCAACTTGCGAATAAATTAACGG
S-T25-R GGGGATCCTCAATCGTACATCAGTTCATCCAGC
W-Down-F GTCGACGTTTTACGAGCACTCGAAGG
W-Down-R AAGCTTGCGGCTGCTTGTTTTTGTTG
W-T18-F CCCTCGAGGATGCCAGATCTGTCGCATAAAG
W-T18-R TCAAGCTTATTAAACCACCTTCCTCAAGAGTTT
W-T25-F GGCTGCAGGGATGCCAGATCTGTCGCATAAAG
W-T25-R GGGGATCCTCATAAACCACCTTCCTCAAGAGT
W-Up-F GAATTCCGCCTTGCACGATAACCCAC
W-Up-R GTCGACTGCGGAGGCTTCATGGCTTA