TABLE 1.

List of selected B. anthracis potential vaccine candidates and virulence factorsa

Protein category and ORF no.Protein nameTIGR's equivalent ORFA2012 strain equivalent ORF (GenBank)
S-layer homology domain proteins
    1SLH amidase_2[+]/BA0872gi|21398829
    2S-layer protein Sap[+]/BA0885gi|21398844
    3S-layer protein (EA1)[+]/BA0887gi|21398845
    4SLH amidase_3[+]/BA0898
    5SLH Transglutaminase/protease-like[+]/BA0981/S-layer protein, putativegi|21398925
    6NEAT domain SLH protein[+]/BA1093/S-layer protein, putativegi|21399019
    7SLH protein[+]/BA1127gi|21399050
    8SLH protein[+]/BA1129gi|21399051
    9SLH protein[+]/BA1130gi|21399052
    10SLH amidase_3[+]/BA1817
    11SLH amidase_4[+]/BA1818gi|21399697
    12SLH AAA ATPase[+]/BA1926gi|21399805
    13SLH protein[+]/BA2315gi|21400193
    14SLH amidase_3[+]/BA2528gi|21400397
    15SLH metallo-beta lactamase[+]/BA2803/S-layer protein, putativegi|21400699
    16SLH protein[+]/BA3332gi|21401209
    17SLH protein[+]/BA3338gi|21401213
    18SLH protein[+]/BA3695
    19SLH amidase_2[+]/BA3737gi|21401593
    20SLH protein[+]/BA5054gi|21402845
Adhesins/lipoproteins/autotransporters:
    21ABC transporter, substrate binding protein[+]/BA0175gi|21398133
    22Lipoprotein_9[+]/BA0314gi|21398260
    23ABC transporter, substrate-binding protein, SAND domain[+]/BA0382gi|21398333
    24Glutamine ABC transporter[+]/BA0642/amino-acid ABC transporter, permease proteingi|21398601
    25Oligopeptide ABC transporter[+]/BA0656gi|21398617
    26Phosphate ABC transporter, phosphate binding protein PstS[+]/BA0715gi|21398678
    27Putative amino-acid binding transporter[+]/BA0855gi|21398810
    28Probable collagen adhesin LPXTG anchored repeat protein[+]/BA0871/LPXTG-motif cell wall anchor domain proteingi|21398828
    29SBP_BAC_5 peptide binding protein[+]/BA1191gi|21399103
    30Spermidine/putrescine ABC transporter[+]/BA1300gi|21399202
    31Periplasmic binding protein-like II[+]/BA1735gi|21399623
    32Periplasmic binding protein-like I ANF-receptor binding domain[+]/BA1931/branched-chain amino acid ABC transportergi|21399809
    33SBP_bac_5 amino-acid binding protein[+]/BA2848/oligopeptide ABC transportergi|21400746
    34Bacterial periplasmic substrate-binding protein sulfur utilization[+]/BA2921/sulfonate ABC transportergi|21400805
    35Putative ABC superfamily (peri_perm) sugar transporter[+]/BA2975gi|21400856
    36PsaA-like protein[+]/BA3189/adhesion lipoproteingi|21401074
    37SPB_BAC_3/alkylphosphonate ABC tranporter[+]/BA3750gi|21401605
    38Fibronectin/fibrinogen-binding protein[+]/BA4013gi|21401859
    39Helical backbone metal receptor/ferrichrome ABC transporter[+]/BA4597gi|21402412
    40Iron(III) dicitrate transporter(fhud)[+]/BA4766/iron compound ABC transporter, iron binding
    41ABC transporter, ATP-binding protein[+]/BA5003
    42Outer membrane protein/Lipoprotein_9[+]/BA5220/ABC transporter, substrate binding protein, putativegi|21397467
    43Probable collagen adhesin anchored repeat protein[−]/BA5258/cell wall surface anchor family proteingi|21397496
    44Iron(III) ABC transporter, periplasmic binding protein[+]/BA5330gi|21397566
    45AfuA-like protein, iron(III) ABC transporter, periplasmic[+]/BA5501/lipoproteingi|21397724
Repeat proteins:
    46Anchored repeat protein[+]/BA0397/LPXTG-motif cell wall anchor domain proteingi|21398347
    47Family similar to phage tail protein[+]/BA0477/tape measure protein, putativegi|21398428
    48Internalin-like protein[+]/BA0552
    49Secreted ANK repeat protein[+]/BA0564gi|21398517
    50Repeat protein[−]/BA0881/hypothetical proteingi|21398840
    51Repeat domain Tol-B C-terminal domain[+]/BA1311/lipoprotein, putativegi|21399212
    52LRR repeat protein SLH protein[−]/BA1346/internalin, putative
    53Bacterial neuraminidase repeat protein[+]/BA1755gi|21399642
    54Fibronectin-binding protein, putative[+]/BA1840gi|21399720
    55Contractile-protein like/enterotoxin A[+]/BA1887gi|21399764
    56Enterotoxin B[+]/BA1888gi|21399765
    57SH3_bac NLP/P60 family protein[+]/BA1952/NLP/P60 family proteingi|21399829
    58Secreted repeat protein[−]/BA2029/hypothetical protein
    59TPR repeat protein[−]/BA2179/hypothetical protein
    60TPR repeat protein[+]/BA2180gi|21400051
    61TPR repeat protein[+]/BA2193
    62Repeat protein[−]/BA3065/hypothetical proteingi|21400947
    63Bacterial neuraminidase repeat protein[+]/BA3445gi|21401316
    64Ankyrin repeat protein, similar to ankyrin repeat domain of bcl-3[+]/BA3478/ankyrin repeat domain proteingi|21401353
    65Large repeat protein[−]/BA3725/conserved repeat domain proteingi|21401582
    66Collagen-like protein[−]/BA3841/conserved hypothetical protein
    67VrrA[−]/BA4510/missing sequence, authentic frameshiftgi|21402333
    68Repeat protein[−]/BA4659/LysM domain protein,gi|21402472
    69Collagen-repeat and CBD domain protein[−]/BA4764/hypothetical proteingi|21402569
    70NEAT-anchored protein[−]/BA4787/conserved hypothetical proteingi|21402590
    71Secreted NEAT protein[−]/BA4788/conserved domain proteingi|21402591
    72Secreted ANK repeat protein[+]/BA4922/ankyrin repeat domain protein
    73Collagen triple-helix domain protein[+]/BA4978/collagen triple helix repeat domain protein
    74VrrB[+]/BA4989
    75Secreted repeat protein[−]/BA5058/lipoprotein, putativegi|21402849
    76Secreted repeat protein[−]/BA5326/lipoprotein, putativegi|21397561
Enzymes
    77Peptidase_S9 prolyl oligopeptidase family[+]/BA0165gi|21398122
    78FAA hydrolase/lyase[+]/BA0241gi|21398183
    79GDSL-like lipase/acetylhydrolase[−]/BA0242/homogentisate 1,2-dioxygenase, putative
    80ALDH-like/delta-1-pyrroline-5-carboxylate dehydrogenase[+]/BA0309gi|21398255
    81Polysaccharide deacetylase-like protein[+]/BA0331gi|21398276
    82Alkyl hydroperoxide reductase, subunit C[+]/BA0345gi|21398291
    83Transglycosylase with Fn3 and cellulose-binding domains[+]/BA0385/chitinase Bgi|21398336
    84Endolysin[+]/BA0485/glycosyl hydrolase, family 25, putativegi|21398435
    85Peptidase M9, collagenase[+]/BA0555gi|21398504
    86PLDc[+]/BA0625gi|21398583
    87Immune inhibitor A metalloprotease[+]/BA0672gi|21398634
    88Phospholipase C/cereolysin[+]/BA0677
    89Sphingomyelinase C[+]/BA0678
    90Putative sortase[+]/BA0688
    91Spore germination protein GerLC[+]/BA0711gi|21398674
    92Purple acid-phosphatase Fn3 and laminin-binding domains[+]/BA0815/purple-acid phosphatasegi|21398776
    93Proline racemase[+]/BA0901gi|21398858
    945-Methycytosine-specific restriction-related enzyme[−]/BA0928/conserved domain proteingi|21398882
    95Phospholipase A2 family protein /PAF acetyl hydrolase[−]/BA0950/conserved hypothetical proteingi|21398901
    96Dihydroxyacetone kinase family protein[+]/BA0965gi|21398915
    97Alcohol dehydrogenase, zinc containing[+]/BA1004gi|21398944
    98α/β-hydrolase[+]/BA1019
    99PrsA[+]/BA1041gi|21398974
    100Isocitrate lyase[+]/BA1132
    101Catalase[+]/BA1159gi|21399079
    102PrsA[+]/BA1169gi|21399086
    103Metalloproteases (“zinctins”) peptidase M3 family[+]/BA1206/oligoendopeptidase Fgi|21399117
    104Probable O-methyltransferase[+]/BA1221gi|21399132
    105Immune inhibitor A[+]/BA1295gi|21399197
    106α/β-hydrolase PhaC(polyhydroxyalkanoic acid synthase)[+]/BA1331gi|21399229
    107PLP-dependent transferases[+]/BA1341/aminotransferase, class Vgi|21399241
    108Peptidase M3 family[+]/BA1353/oligoendopeptidase F, putative
    109Diaminopimelate decarboxylase[+]/BA1438gi|21399334
    110Secreted peptidase M23/M37 LysM domain[+]/BA1500/peptidase, M23/M37 family
    111Amidase 2/autolysin[+]/BA1514gi|21399412
    112Putative metallo-dependent phosphatase/CapA[−]/BA1855/conserved hypothetical protein
    113α/β Hydrolase[+]/BA1860gi|21399739
    114Putative lipase/haloperoxidase[−]/BA1866/conserved hypothetical proteingi|21399746
    115Secreted peptidase, M23/M37[+]/BA1879
    116Multicopper oxidase/Cupredoxins[+]/BA1902gi|21399781
    117Secreted peptidase, M23/M37 with SH3b domains[+]/BA1903/peptidase, M23/M37 familygi|21399782
    118PLDc[+]/BA2054gi|21399928
    119ScdA[+]/BA2147
    120Aspartate/glutamate racemase[+]/BA2223gi|21400101
    121Had-like hydrolase[+]/BA2231gi|21400110
    122Penicillin-binding protein 2x and transpeptidase[+]/BA2238
    123Secreted FtsK/SpoIIIE family protein[+]/BA2321gi|21400199
    124Dependent dsDNA exonuclease SbcC[+]/BA2360/exonuclease, SbcC family, putative
    125Inosine-uridine preferring nucleoside hydrolase family[+]/BA2400gi|21400272
    126Secreted Metallo-hydrolase/oxidoreductase betalactamase[+]/BA2472gi|21400341
    127P-loop containing nucleotide triphosphate hydrolase family[−]/BA2556/hypothetical proteingi|21400433
    128α/β-hydrolases/lipase[+]/BA2607gi|21400493
    129Metallo-hydrolase/oxidoreductase[−]/BA2688/conserved hypothetical proteingi|21400582
    130Aromatic-l-amino-acid decarboxylase[+]/BA2724/decarboxylase, pyridoxal-dependentgi|21400621
    131Secreted metalloproteinase/thermolysin-like[−]/BA2730/neutral proteasegi|21400627
    132Spore cortex lytic enzyme[+]/BA2748gi|21400649
    133Kynureninase (PLP dependent transferase)[+]/BA2753gi|21400654
    134Endolysin [Phage bastille][+]/BA2805/glycosyl hydrolase, family 25, putativegi|21400701
    135Putative diaminopimelate epimerase[−]/BA2833/hypothetical proteingi|21400729
    136B-cell mitogen[+]/BA2834gi|21400730
    137X-Pro dipeptidyl-peptidase (S15 family)/cocaine esterase[+]/BA2860/hydrolase, CocE/NonD familygi|21400756
    138Fumble protein/Panthetoate kinase[−]/BA2901/conserved hypothetical proteingi|21400792
    139Putative glutathionylspermidine synthase[+]/BA2932
    140Polysaccharide deacetylase[+]/BA2944gi|21400827
    141Secreted SH3b domain protein, putative metallohydrolase[−]/BA2967/conserved domain proteingi|21400849
    142Histidinol-phosphate aminotransferase[−]/BA2995/pyrroline-5-carboxylate reductase
    143Alkaline phosphatase[+]/BA3001gi|21400884
    144Peptidase[+]/BA3033gi|21400917
    145Probable methyl transferase[−]/BA3085/conserved hypothetical proteingi|21400965
    146Pyrrolidone carboxyl peptidase[+]/BA3090gi|21400970
    147Aminoglycoside phosphotransferase[+]/BA3111gi|21400988
    148Pyrroline-5-carboxylate reductase[+]/BA3143gi|21401019
    149Isochorismatase family protein[+]/BA3166gi|21401049
    150Isochorismatase family protein[+]/BA3167gi|21401050
    151Lactamase class C domain (PBPX family)[+]/BA3214/penicillin-binding protein, putativegi|21401102
    152Metallo-hydrolase/oxidoreductase[−]/BA3247/conserved hypothetical proteingi|21401131
    1533-beta hydroxysteroid dehydrogenase/isomerase family protein[+]/BA3248gi|21401132
    154Secreted peptidase_P9 (collagenase) PKD domain[+]/BA3299
    155Isochorismatase family protein[+]/BA3315gi|21401191
    156Secreted beta-lactamase/d-ala carboxypeptidase[+]/BA3346/6-aminohexanoate-dimer hydrolase, putativegi|21401221
    157Perfringolysin O[+]/BA3355/thiol-activated cytolysingi|21401229
    158Protein-tyrosine phosphatase-like protein[+]/BA3394gi|21401268
    159Peptidase M4 (metalloproteinase)[−]/BA3442/neutral protease
    160Viral enhancin [metalloproteinase][+]/BA3443/enhancin family proteingi|21401314
    161Cyclopropane-fatty-acyl-phospholipid synthase[+]/BA3460gi|21401332
    162Metallo-beta-lactamase family protein[+]/BA3514gi|21401388
    163Metalloprotease family protein[+]/BA3553/oligoendopeptidase Fgi|21401418
    164Microbial collagenase PKD domain[+]/BA3584
    165Oxidoreductase family NAD binding Rossman fold[+]/BA3655/oxidoreductase, Gfo/Idh/MocA familygi|21401519
    166Serine protease PDZ domain[+]/BA3660/serine proteasegi|21401523
    167Anaerobic ribonucleotide triphosphate reductase phage[+]/BA3663gi|21401527
    168Spore peptidoglycan hydrolase with LysM domains[+]/BA3668/Glycosyl hydrolase, family 18gi|21401531
    169Amidase_2 and peptidoglycan recognition domain[+]/BA3698/N-acetylmuramoyl-l-alanine amidasegi|21401556
    170Amidase_2 with SH3b domains[+]/BA3767/N-acetylmuramoyl-l-alanine amidase, family 2
    171Secreted exochitinase Chi36[+]/BA3854gi|21401702
    172α/β-hydrolase[+]/BA3877gi|21401726
    173Phosphoinositol specific phospholipase C[+]/BA3891/1-phosphatidylinositol phosphodiesterasegi|21401737
    174Secreted peptidase S8 (subtilisin-like)[+]/BA3892
    175Secreted LysM repeats protein[+]/BA3893/cell wall hydrolase, putativegi|21401739
    176Thermostable dipeptidase (Zn dependent)[+]/BA3911/renal dipeptidase family proteingi|21401756
    177Amidase 2[+]/BA4073gi|21401919
    178Clp protease family protein[+]/BA4092gi|21401939
    179Carbon-nitrogen hydrolase[+]/BA4253gi|21402087
    180Peptidyl-prolyl cis-trans isomerase (PPlase)[+]/BA4283/peptidyl-prolyl cis-trans isomerase, cyclophilin-tygi|21402116
    181Metallo-dependent phosphatase secreted and LPXTG anchored[+]/BA4346/2′,3′-cyclic-nucleotide 2′-phosphodiesterasegi|21402178
    182Prolyl 4-hydroxylase alpha subunit homologue[+]/BA4459/prolyl 4-hydroxylase-alpha subunit domaingi|21402285
    183Pterin-4a-carbinolamine dehydratase[+]/BA4587gi|21402401
    184FKBP-like prolyl isomerase[+]/BA4705/trigger factorgi|21402519
    185Class I glutamine amidotransferase-like protein[+]/BA4720/ThiJ/PfpI family proteingi|21402528
    186Spore cortex lytic enzyme[+]/BA3668/Glycosyl hydrolasegi|21401531
    187Metallo-dependent hydrolase/putative deaminase[+]/BA4727/N-acyl-d-amino-acid deacylase family
    188Secreted oxidoreductase/putative acid phosphatase[+]/BA4746/acid phosphatasegi|21402552
    189d-Alanyl-d-alanine-carboxypeptidase family protein[+]/BA4750gi|21402556
    190Lysophospholipase homolog[−]/BA5009/conserved hypothetical proteingi|21402804
    191LPXTG-site transpeptidase family protein[+]/BA5069
    192Cu,Zn superoxide dismutase secreted[+]/BA5139gi|21397388
    193Phosphoglycerate mutase-like protein[+]/BA5203gi|21397451
    194Acid phosphatase[+]/BA5238/PAP2 family proteingi|21397479
    195Secreted peptidase M4 bacillolysin[+]/BA5282/neutral protease Bgi|21397519
    196NLP/P60 family peptidase M27/37[+]/BA5427/endopeptidase LytE, putativegi|21397656
    197UDP-N-acetyl-d-mannosamine dehydrogenase Cap5O[+]/BA5512
    198Heparinase III protein homolog[−]/BA5513/conserved domain protein
    199NADH dehydrogenase I, H subunit[+]/BA5538gi|21397767
    200Cell-wall associated hydrolase[+]/BA2849/NLPP60 family proteingi|21400747
    201Secreted phospholipase D/nuclease[+]/BA5591/cardiolipin synthase domain proteingi|21397822
    202UDP-glycosyltransferase/glycogen phosphorylase[+]/BA5670/glycosyl transferase 1, group 1gi|21397904
    203Methyl-accepting chemotaxis protein[+]/BA5673gi|21397907
    204Secreted metalloprotease[+]/BA5706/oligoendopeptidase F, putativegi|21397939
Others
    205Secreted putative α/β-hydrolase[−]/BA0177/lipoprotein, putativegi|21398135
    206Transglycosylase[−]/BA0543/penicillin-binding domain proteingi|21398490
    207Outer surface protein[−]/BA0789/conserved hypothetical protein
    208Outer surface protein[−]/BA0795/conserved hypothetical proteingi|21398756
    209Sensor histidine kinase/response regulator[+]/BA0996gi|21398939
    210Siderophore biosynthesis protein[+]/BA1981gi|21399857
    211Large anchored repeat protein with FN3 and CBM domains[+]/BA1094/wall-associated protein, putativegi|21399020
    212Wall-associated domain protein[+]/BA1098
    213PlcR[+]/BA1163/DNA-binding protein
    214Spore coat-associated protein, putative[+]/BA1290gi|21399194
    215Flagellar motor switch protein, putative/SpoA[+]/BA1662gi|21399552
    216Flagellar hook-associated FliD[+]/BA1671gi|21399562
    217Flagellar basal-body rod protein[+]/BA1674/FlgBgi|21399565
    218FligG/C[+]/BA1679/flagellar motor switch protein FliGgi|21399570
    219Similar to flagellar hook assembly protein[+]/BA1685gi|21399576
    220Flagellin/IL-4-like[+]/BA1706gi|21399593
    221SpoA/flagellin B chain[−]/BA1710/flagellar motor switch protein FliN, putativegi|21399598
    222Microcin immunity protein MccF[+]/BA1949gi|21399826
    223Bacterial SH3 domain homolog[+]/BA5481/conserved domain proteingi|21397703
    224Siderophore biosynthesis protein[+]/BA1982gi|21399858
    225ErfK family protein[−]/BA3232/conserved hypothetical proteingi|21401118
    226LPXTG-anchored coiled coil protein[+]/BA3254/LPXTG motif cell wall anchor domain proteingi|21401137
    227Secreted repeat protein[+]/BA3366/lipoprotein, putativegi|21401240
    228LPXTG-anchored repeat protein similar to VLPE prolipoprotein[+]/BA3367/LPXTG motif cell wall anchor domain proteingi|21401241
    229TPR repeat/HTH 3 protein[+]/BA3373/DNA-binding proteingi|21401248
    230Toxin secretion/phage lysis holin[+]/BA4074gi|21401920
    231Wall-associated domain protein[+]/BA5303/Conserved domain proteingi|21397539
    232CheY-homologous receiver domain/spo0A[+]/BA4394/stage 0 sporulation protein Agi|21402223
    233Wall-associated domain protein[+]/BA4742gi|21402548
    234NEAT domain anchored protein[−]/BA4789/LPXTG motif cell wall anchor domain proteingi|21402592
    235HlyD family secretion protein, probably MDR protein[−]/BA4813/conserved hypothetical proteingi|21402613
    236PhoR[+]/BA4832/sensory box histidine kinase PhoRgi|21402631
    237Cell surface anchor family protein[+]/BA5070gi|21402863
    238Putative membrane protein[+]/BA5324gi|21397559
    239VWA[−]/BA0623/hypothetical proteingi|21398581
    240Probable hemolysin[+]/BA5701/channel protein, hemolysin III familygi|21397934
  • a Putative candidate vaccine ORFs are grouped in categories, as described in the text. Preliminary functional annotation of the chromosome draft sequence-derived ORF products, is compared to the complete chromosome homologs (78). For easy future reference, ORF products are numbered sequentially (rather than by contig allocation, column 1) and listed side by side with draft genome-based annotation (column 2). Agreement between the chromosome draft sequence-based annotation and TIGR's data is marked in column 3, where identical annotations are marked as [+]. In cases of either discrepancy or disagreement in annotation (marked as [−]), TIGR's annotation is provided as well (column 3). The gi numbers of the A2012 strain equivalent ORF products are given in column 4 (79).