TABLE 1.

Development of inflammatory infiltrates and tissue injury in the bovine and murine ileal mucosa following inoculation with the serotype Typhimurium wild type, the sipA sopABDE2 mutant, or sterile LB broth

Loop sourcePathological changeLoop inoculated withAvga qualitative score of pathological changesb in tissue collected at the following times (h) after inoculation of loops
0.51248
CalvesTissue-associated PMN infiltrationWild type1.72.73.32.74.0
sipA sopABDE2 mutant1.71.32.71.72.3
LB broth2.02.02.01.31.0
Intravascular PMN infiltrationWild type0.71.72.31.32.3
sipA sopABDE2 mutant1.00.72.32.01.7
LB broth1.00.51.31.31.0
Monocyte infiltrationWild type2.01.71.72.33.0
sipA sopABDE2 mutant1.91.32.31.72.0
LB broth1.72.01.71.71.3
Detachment of surface epitheliaWild type0.01.01.41.73.3
sipA sopABDE2 mutant0.70.32.01.01.3
LB broth0.01.41.00.30.7
MiceTissue-associated PMN infiltrationWild type0.00.00.30.00.3
sipA sopABDE2 mutant0.00.30.70.71.3
LB broth1.00.00.00.00.3
Intravascular PMN infiltrationWild type0.50.71.01.01.0
sipA sopABDE2 mutant0.31.02.33.02.7
LB broth1.01.00.70.01.3
Monocyte infiltrationWild type1.53.03.33.03.5
sipA sopABDE2 mutant2.02.31.32.32.3
LB broth1.71.01.70.72.3
Detachment of surface epitheliaWild type0.00.72.02.04.8
sipA sopABDE2 mutant0.30.30.00.01.0
LB broth0.00.00.00.00.7
  • a Each data point represents the average score from three independent experiments.

  • b The histopathological changes of murine and bovine Peyer's patches were assessed in a blinded manner by two veterinary pathologists and independently scored on a scale of 0 to 5, with 5 being the highest level of histologic changes.