TABLE 3.

Activities of hemagglutination, biofilm formation, and hemolysis for P. gingivalis ATCC 33277 and gingipain-deficient mutants

StrainHemagglutinationa (assay 1/assay 2)Biofilm formationb (A592)Hemolysisc (A540)
ATCC 332778/160.35 ± 0.011.1 ± 0.15
KDP131 (rgpA)4/80.56 ± 0.03*NDd
KDP132 (rgpB)8/160.32 ± 0.01ND
KDP129 (kgp)2/40.36 ± 0.011.76 ± 0.05*
KDP112 (rgpA rgpB)0.28 ± 0.020.73 ± 0.12
KDP128 (rgpA rgpB kgp)0.21 ± 0.010.83 ± 0.09
  • a Reciprocal of the highest dilution producing hemagglutination. −, negative.

  • b Means ± standard deviations of four assays. The asterisk indicates a significant difference with respect to the wild-type strain (P < 0.005).

  • c Complete lysis of erythrocytes caused by adding 1% SDS gave an A540 of 1.8. The mean baseline value of the assay was 0.048. The asterisk indicates a significant difference with respect to the wild-type strain (P < 0.005).

  • d ND, not determined.