TABLE 1.

Production of proteolysis zones on a gelatin plate and uptake of radiolabeled amino acids by P. gingivalis ATCC 33277 and gingipain-deficient mutants

StrainProteolysis zone (mm)aRadioactivity incorporated (DPM)b
ATCC 332779 ± 0.526,522 ± 1,217
KDP131 (rgpA)9 ± 0.548,696 ± 1,522*
KDP132 (rgpB)12 ± 145,652 ± 739*
KDP112 (rgpA rgpB)3 ± 1*54,348 ± 3,043*
KDP129 (kgp)11 ± 0.552,391 ± 1,323*
KDP128 (rgpA rgpB kgp)0*66,087 ± 870*
  • a Zones were measured from the edge of the bacterial growth to the outer margin of the clear zone. Shown are means ± standard deviations of three independent experiments. The asterisks indicate a significant difference with respect to the wild-type strain (P < 0.005).

  • b Means ± standard deviations of three independent experiments. The asterisks indicate a significant difference with respect to the wild-type strain (P < 0.005).