TABLE 2.

Adherence to human cells and lipolytic activities of recombinant E. coli cells

ConstructMcaP expressionAdherence to cell lineaLipolytic activityb
ChangA54916HBE14o
pELCCF10.2 ± 0.030.06 ± 0.01NDe
pELFOS190+10.4 ± 2.9c9.0 ± 1.6cND+
pELFOS190TN60.2 ± 0.040.1 ± 0.02ND
pBR3220.2 ± 0.030.2 ± 0.060.1 ± 0.05
pJTmcaP+22.3 ± 3.7d4.9 ± 1.4d4.9 ± 2.1d+
  • a Adherence is expressed as the percentage of bacteria binding to monolayers (± standard error).

  • b As determined by the presence of halos surrounding bacteria streaked onto LB medium-1% Tween 80 agar plates.

  • c The difference in the P value compared to the value for the negative control pELCCF1 was found to be statistically significant using a Mann-Whitney test.

  • d The difference in the P value compared to the value for the negative control pBR322 was found to be statistically significant using a Mann-Whitney test.

  • e ND, not determined.