TABLE 3.

Expression profile of chemokine genes in bovine and murine ileal Peyer's patches determined by RT-PCR

ChemokineLoop inoculated withAvg.a intensity of RT-PCR productsb from tissue collected at the following times (h) after inoculation of loops
0.51248
Bovine GROαWild type21,65943,86239,52541,39844,374
sipA sopABDE2 mutant1,6817,3088,59522,76510,401
LB broth2,3663,2569243,0193,658
Bovine GROγWild type12,73643,33144,57435,69143,014
sipA sopABDE2 mutant6,15912,78030,28537,51723,707
LB broth3,2025,6223,2134,1879,227
Bovine IL-8Wild type25,83938,27237,42837,21336,497
sipA sopABDE2 mutant19,98828,17525,75138,65731,395
LB broth6,43012,31411,37117,23911,525
Bovine GCP-2Wild type20,90630,23828,73538,99834,338
sipA sopABDE2 mutant22,66623,16232,72431,32536,786
LB broth15,02514,58711,33110,29118,894
Bovine MCP-1Wild type19,86534,80729,24223,86827,201
sipA sopABDE2 mutant5,5667,48216,17816,82411,345
LB broth3,6163,8462,5061,153772
Bovine MCP-2Wild type38,73849,54544,09644,12952,014
sipA sopABDE2 mutant21,10730,19238,42749,68043,826
LB broth20,51122,79522,41520,62219,531
Bovine MIP-1αWild type3,1504,7116,0195,8245,353
sipA sopABDE2 mutant7,2164,8173,6996,5245,791
LB broth2,0674,0964,9772,3302,483
Bovine RANTESWild type41,90742,04242,01834,21939,325
sipA sopABDE2 mutant46,13543,18443,06439,05438,471
LB broth32,05841,61539,26530,62029,846
Murine KCWild type47,63652,14850,81252,74453,246
sipA sopABDE2 mutant40,68850,86650,59451,20849,622
LB broth18,58142,70139,42150,57940,688
Murine GCP-2Wild type8,61616,65547,26454,46856,123
sipA sopABDE2 mutant6,8786,35941,98452,23748,917
LB broth4,7385,68915,31545,89539,984
Murine MIP-2Wild type37,23252,47151,69851,40356,766
sipA sopABDE2 mutant26,21552,04955,77756,67344,451
LB broth15,54633,14040,27956,93144,342
Murine JEWild type28,37027,81428,59230,70633,599
sipA sopABDE2 mutant7,68614,96913,4646,4016,417
LB broth12,96015,75612,34415,92717,240
Murine MCP-2Wild type12,24338,36245,16443,51049,735
sipA sopABDE2 mutant1,52825,61443,67848,95335,019
LB broth1,73217,72727,71330,07126,441
Murine MIP-1αWild type14,19626,05735,65139,37938,554
sipA sopABDE2 mutant18,24013,43627,96829,01818,001
LB broth8,58019,39610,92016,76016,433
Murine RANTESWild type29,99440,02135,28931,55740,429
sipA sopABDE2 mutant35,29326,34926,70832,24142,878
LB broth32,05419,46432,94640,77638,599
  • a Each data point represents the average of three independent experiments.

  • b Intensity of bands was determined using the NIH image software.