TABLE 2.

Percent expression of CD45RO and CD26 on CD4+ cells from M. avium subsp. paratuberculosis- infected cattle upon antigen stimulation

Subseta% Expression
Freshly isolatedMediumPPDaWCS
CD45RO42.3 (2.85)52.3 (3.18)4.0 (0)***3.0 (0.58)***
CD45RO+56.7 (2.33)45.3 (2.60)95.3 (0.67)***97.0 (1.00)***
CD2670.3 (1.86)89.7 (2.73)19.5 (6.5)**30.3 (11.85)*
CD26+29.3 (2.19)7.3 (3.38)80.5 (6.50)**69.3 (12.17)*
  • a At 286 days postchallenge, PBMC were cultured with either medium alone, 4 μg of PPDa/ml, or 20 μg of strain K10 WCS/ml for 7 days, harvested, and analyzed by flow cytometry. Data represent CD26 or CD45RO expression [mean percent positive or negative (± SEM)] by CD4+ cells. *, P < 0.05; **, P < 0.01; ***, P < 0.001; differs from response to media alone and expression by freshly isolated PBMC, same subset (i.e., horizontal comparisons).