TABLE 2.

Oligonucleotides used in this study

OligonucleotideaSequence (5′-3′)Usage
E54.1 AATGGTATGTTCAATGACC Expression
E54.2 AGAAGCACGCGAACAAGCA Expression
E54.3 TCTGCTTGTTCGCGTGCTT Expression
E54.4 CACCATTCACACTTCCAGA Expression
E54.5 CATGCTGACAGCAAAGTCA Expression
Eh54.1 GTCGGAGGAATGAAGAGT Disruption
Eh54.3 GTTGACGAGCTTGTTCTG Disruption
sagAup CGGAATTCGAAATGCTAGACTTAACGC Complementation
sagAdown CGGAATTCGCAAGCAGGTATCGACATG Complementation
  • a E54.1 and E54.5 were used to express the full-length SagA (F). E54.1 and E54.3 were used to express the N-terminal domain of SagA (N). E54.1 and E54.4 were used to express the N-terminal half of SagA (containing the N-terminal and middle domains [NM]). E54.2 and E54.4 were used to express the middle (repeat) domain of SagA (M). E54.2 and E54.5 were used to express the C-terminal half of SagA (containing middle and C-terminal domains [MC]). Letter codes correspond to lane designations in Fig. 5.