TABLE 1.

Strains and plasmids

Plasmid or StrainRelevant propertiesaReference or source
Plasmids
    pAT18Plasmid vector; Ermr45
    pQE31His6 expression vector; AmprQiagen
    pTEX4577Plasmid vector, derivative of pBluescript KS(−); Kanr39
    pTEX5235Plasmid vector, derivative of pBluescript KS(−); Spcr43
    pTEX5236Cosmid vector, derivative of pBeloBAC11; Camr37
    43
    pTEX10000pTEX5235 derivative expressing N-terminal part of SagAThis study
    pTEX10001pTEX5235 derivative expressing C-terminal part of SagAThis study
    pTEX10002pTEX5236 derivative expressing full-length SagAThis study
    pTEX10003pAT18 derivative expressing SagAThis study
Strains
    TX16 E. faecium endocarditis isolate (Houston, Tex.)1
    TX1330 E. faecium community-derived fecal isolate (Houston, Tex.)38
    TX10003TX1330 with pTEX10003This study
    TX10004TX10003 with a disrupted chromosomal sagAThis study
    448 E. faecium blood isolate (Poland)This study
    DH5α E. coli strainQiagen
    d1-27 (TX10000)DH5α with plasmid expressing N-terminal part of SagAThis study
    d1-29 (TX10001)DH5α with plasmid expressing C-terminal part of SagAThis study
    D7A3 (TX10002)DH5α with cosmid expressing whole-length SagAThis study
    M15 (pREP4)Host for pQE31; derivative of E. coli K-12 with plasmid pREP4 expressing lac repressor; KanrQiagen
  • a Spc, spectinomycin; Cam, chloramphenicol; Amp, ampicillin; Kan, kanamycin; Erm, erythromycin; superscript “r,” resistance.