TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionReference(s)
S. aureus
    8325-4Prophage-cured strain of NCT8325, harboring an 11-bp deletion in rsbU, which regulates sigB activity by activating rsbV, a factor that competitively binds to the anti-sigma factor RsbW39, 42
    RN4220Mutant strain of 8325-4 that accepts foreign DNA39
    RN6390 agr + laboratory strain related to 8325-439
    RN6911 agr mutant of RN6390 (Δagr::tetM)42
    SH10008325-4 with intact rsbU22
    ALC1905 sarT mutant of RN6390 (sarT::ermC)48
    ALC1927 sarS mutant of RN6390 (sarS::ermC)15
    ALC2009ALC1927 with pSK236 containing a 1.2-kb sarS coding region fragment17
    ALC2056 agr sarT mutant of RN639048
    ALC2057RN6390 sarA::kan48
    ALC2071ALC1905 with pALC204748
    ALC2076ALC2056 with pALC204748
    ALC2122ALC2057 with sarT::ermC (sarA sarT)48
    ALC2150ALC2122 with pALC204748
    ALC2221ALC1905 with pALC2217This study
    ALC2235ALC2056 with pALC2217This study
    ALC2236ALC2122 with pALC2217This study
    ALC2253ALC2122 with pALC2073This study
    ALC2254ALC2056 with pALC2073This study
    ALC2255ALC1905 with pALC2073This study
    ALC2305RN4220 with pALC2229This study
    ALC2313RN4220 sarT::tetKThis study
    ALC2389 sarT mutant of SH1000 (sarT::tetK)This study
    ALC2394ALC2389 with pALC2047This study
    ALC2398RN6390 with pALC2047This study
    ALC2399ALC2389 with pALC2217This study
    ALC2402ALC2389 with pACL2073This study
Plasmids
    pALC1894pUC18 with 3.2-kb fragment containing the sarT coding region48
    pALC2047pSK236::sarT48
    pALC2073pSK236::xyl/tetO promoter::tetR3
    pALC2217 sarT ligated into the EcoRI and SstI sites of pALC2073This study
    pALC2223pALC1894 containing the sarT mutation (sarT::tetK)This study
    pALC2229pBT2 with the sarT mutation (sarT::tetK)This study
    pACL2321pCR2.1 Topo with the sarS promoter fragmentThis study