TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionaSource or reference
Strains
    V. vulnificus
        MO6-24/OClinical isolate47
        CMCP6Clinical isolateChonnam National University Hospital
        CMCP98KClinical isolateChonnam National University Hospital
        CMM1701CMCP6 with the insertion mutation in Vv-ive-1 gene; CmrThis study
        CMM1702CMCP6 with the insertion mutation in Vv-ive-2 gene; CmrThis study
        CMM1703CMCP6 with the insertion mutation in Vv-ive-3 gene; CmrThis study
        CMM1704MO6-24/O with the insertion mutation in Vv-ive-4 gene; CmrThis study
        CMM1705CMCP6 with the insertion mutation in Vv-ive-5 gene; CmrThis study
        CMM1706CMCP6 with the insertion mutation in Vv-ive-6 gene; CmrThis study
        CMM1707CMCP6 with the deletion mutation in Vv-ive-7 geneThis study
        CMM1708MO6-24/O with the deletion mutation in Vv-ive-8 geneThis study
        CMM1709MO6-24/O with the insertion mutation in Vv-ive-9 gene; CmrThis study
        CMM1710CMCP6 with the deletion mutation in Vv-ive-10 geneThis study
        CMM1711CMCP6 with the insertion mutation in Vv-ive-11 gene; CmrThis study
        CMM1712CMCP6 with the insertion mutation in Vv-ive-12 gene; CmrThis study
        CMM1715CMM1710 containing pCMM1701; Ampr TcrThis study
    E. coli
        BL21(DE3)F ompT hsdSB(rB mB) gal dcm (DE3)Novagen
        DH5αF φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK mK+) phoA supE44 λ thi-1 gyrA96 relA1Laboratory collection
        SY327 λpirΔ(lac pro) argE(Am) rif nalA recA56 λpir lysogen39
        SM10 λpirthi thr leu tonA lacY supE recA::RP4-2-Tcr::Mu λpirR6K lysogen; Kmr39
Plasmids
    pET30a,b,cInducible-expression vectors carrying N-terminal His · Tag/thrombin/S · Tag/enterokinase configuration, providing three different reading frames at the multicloning site; KmrNovagen
    pDM4A suicide vector with ori R6K sacB, Cmr40
    pLAFR3IncP cosmid vector; Tcr54
    pLAFR3IIpLAFR3 with bla inserted at the cos siteThis study
    pRK2013IncP Kmr Tra Rk2+ repRK2 repE19
    pCMM1701pLAFR3I1 with hlyU ORFThis study
    pUTKm1Tn5-based insertion delivery plasmid; Ampr23
  • a Cmr, CHL resistance; Tcr, TET-resistance; Kmr, KAN resistance; Ampr, AMP resistance.