TABLE 3.

Influence of tlr4 and MHC-II genes on peritoneal macrophage nitrite secretion after infection with E. chaffeensisa

DayNitrite secretionb (μM) in mice with tlr4 genotypeNitrite secretionb (μM) in mice with MHC-II genotype
n/n (n = 10)d/d (n = 10)P+/+ (n = 8)−/− (n = 12)P
310 (0, 29)0 (0, 0)<0.060 (0, 5)0 (0, 23)NS
887 (65, 100)55 (37, 77)<0.0590 (71, 99)57 (36, 79)<0.06
16143 (120, 155)24 (16, 64)<0.0190 (50, 132)66 (20, 144)NS
23105 (55, 120)15 (7, 24)<0.0137 (22, 66)41 (10, 107)NS
3040 (25, 77)23 (0, 43)<0.113 (0, 38)41 (30, 58)NS
5148 (2, 83)16 (0, 54)NS0 (0, 9)68 (8, 84)NS
9332 (0, 92)0 (0, 7)NS0 (0, 8)54 (0, 112)NS
  • a Peritoneal exudate cells were recovered from mice at the indicated times after i.p. infection with E. chaffeensis and were cultured in vitro for 20 h. Supernatants were assayed for nitrite using the Greiss reagent.

  • b Values are median nitrite concentrations. First and last quartile values are presented in parentheses. Statistical comparisons between functional and nonfunctional alleles were performed at each gene using the Mann-Whitney test. NS, not statistically significant, P > 0.1, except where indicated.