TABLE 1.

Strains and plasmids used in this study

Strain or plasmidGenotype or sequenceReference or source
Strains
    L. pneumophila
        AA1003
        GN229Kanr6
        GR159Kanr6
        GL208Kanr6
        GP247Kanr6
        GP263Kanr6
        AA100kmTAA100; icmT:: KanrThis study
        AA100kmSAA100; icmS:: KanrThis study
         φp24ΔRtxA13, 14
        NU259130b; LpsG::Kanr30
        NU243130b; PilD::Kanr28
        ribrev1This study
        ribrev2This study
        ribrev3This study
    E. coli DH5αsupE44 ΔlacU169 (ψ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1BRL
Plasmids
    pBC SK+ColE1 origin, lacZα, CmrStratagene
    pMW100pMMB207αβ; Cmr; icmGCDJB, tphA, and icmF region29
    pGS-LC-32pMMB207αβ; Cmr; icmTSRQPO34
    pGS-LC-47pMMB207αβ; Cmr; icmNMLKEG33
    pTSRQpBC SK+; Cmr; icmTSRQThis study
    pTSpBC SK+; Cmr; icmTSThis study
    pTpBC SK+; Cmr; icmT geneThis study
    pkanTpBC SK+; Cmr; ; icmT:: KanrThis study
    pSpBC SK+; Cmr; icmS geneThis study
    pkanSpBC SK+; Cmr; ; icmS:: KanrThis study
    pRpBC SK+; Cmr; icmR geneThis study
    pkanRpBC SK+; Cmr; icmR:: KanrThis study
    pQpBC SK+; Cmr; icmQ geneThis study
    pkanQpBC SK+; Cmr; icmQ:: KanrThis study