TABLE 1.

The FACS-positive set of proteinsa

TABLE 1.
  • a Gene identification (ID) in the CWL029 genome (26) and the last four digits of PID protein database accession numbers (427-NNNN) are reported.Two ORF products not included in the PID database are identified by arbitrary codes (shown in parentheses and italics). Theoretical molecular masses (in kilodaltons) were calculated for putative mature forms. The WB analysis column summarizes the results obtained by probing total EB proteins with antisera against the recombinant proteins; numbers in parentheses refer to the blot images shown in Fig. 2. WB results are classified as follows: C, consistent (the predominant band is consistent with the expected molecular weight; additional minor bands may also be present); PC, partially consistent (a band of the expected molecular weight is present together with additional bands of higher molecular weight or greater intensity); NC, nonconsistent (the detected bands do not correspond to predictable molecular weight values); N, negative (no band pattern obtained). FACS results are reported as K-S scores. The 28 proteins which have been detected in C. pneumoniae extracts by 2DE mapping and MALDI-TOF analysis are color highlighted as follows: red, proteins detected both in this work and by Vandahl et al. (54); yellow, a protein reported only in reference 54; green, proteins detected only in this work.