TABLE 2.

Primers used in this study

PrimerSequenceaRestriction enzyme
P1 (salIIcmTS)ACGCGTCGACCACAGTTAAAACTTCAAGCTGAACCSalI
P2 (notIRQ2)ATAAGAATGCGGCCGCTGCTCAGAGCTATTTTTNotI
P3CTAGCTAGCACACTTGCTAATATCTCGCTNheI
P4CTAGCTAGCCGTTTGTTATGTATGACGGNheI
P5CTAGCTAGCCCAGTGGCTCGTTGCTNheI
P6CTAGCTAGCGGTTCAGTTAGATCTGCTAGNheI
P7ACGCGTCGACATGAGCTTATTCAAGGGGGCSalI
P8ATAAGAATGCGGCCGCGGGAACCAAGAATTAGGGGNotI
P9ACGCGTCGACAATGTTTGGTGCTGCTGAATCSalI
  • a Underlined letters are the sequences of restriction enzymes used for cloning.