TABLE 2.

Proteins identified by MALDI-TOFd

Current annotationKalman IDPID ID 437-NNNNNo. of matching peptidesCoveragea (%)Theor. pITheor. MWGRAVY valuecm/z and deduced sequence for peptide selected for PSD analysisc
Pmp_2CPn0013627020305.8487.5−0.220
Conserved outer membrane lipoprotein proteinCPn0278655220666.4928.4−0.162
VaeT omp85 homologCPn0300657618297.6986.0−0.355
OmpH-like OMPCPn0301657710584.7517.3−0.7781607.01 156TTEIIAILNESYFKK169
LcrE (low calcium response E)CPn0324660213394.9843.4−0.468
PepA (leucyl aminopeptidase A)CPn0385666414285.9554.5+0.012
Pmp_6CPn0444672788b5.31142.9−0.187
Pmp_7CPn0445672819225.73100.0−0.113
Pmp_8CPn0446672913215.1694.8−0.170
Pmp_10CPn0449 and -5026325.2294.5−0.143
Pmp_11CPn0451673315215.9197.1−0.203
Pmp_13CPn0453673617246.55100.1−0.116
Pmp_14CPn0454673718236.76101.2−0.036
Pmp_16CPn0467675211136.04102.1−0.273
Art J (arginine binding periplasmic protein)CPn048267679385.4529.1+0.0501471.91 192VVLKDFPNLVATR204
Hypothetical proteinCPn0498678415395.5532.9−0.244
DnaK (heat shock protein)CPn0503679012164.9971.3−0.394
Pmp_20CPn0540683018125.36177.4−0.191
OmcB (60-kDa cysteine-rich OMP)CPn0557684923475.6257.3−0.116
FliY (glutamine binding protein)CPn060469008247.6628.0+0.0981296.78 201IISKPLNADGLR212
5.725.30.056
Mip (FKBP-type peptidyl-prolyl cis-trans isomerase)CPn066169603135.2225.6−0.488914.78 58TFGHLLAR65
OmpA (MOMP)CPn0695699813486.1339.3−0.037
CHLPN 76-kDa protein homolog_1 (CT622)CPn0728703318364.8568.2−0.351
Hypothetical proteinCPn079771086215.1635.9−0.1811398.77 128EGYTHAFVFDGR139
Eno (enolase)CPn0800711114324.6646.1−0.079
PorB (OMP analog)CPn085471701023b5.1034.4−0.048
Pmp_21CPn09637287137b4.84167.3−0.175
HtrA (DO serine protease)CPn0979730612226.3950.1−0.063
  • a Calculated from the whole protein.

  • b Peptides from the C-terminal part of the protein.

  • c GRAVY, grand average of hydropathicity (available at http://www.expasy.ch/tools/protparam.html; PSD, postsource decay.

  • d The results were obtained from protein spots in the 2DE map shown in Fig. 3 and other maps obtained by isoelectrofocusing, at different pH ranges.