TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionReference
S. aureus strains
    RN4220Heavily mutagenized strain that accepts foreign DNA, agr-deficient24
    RN6390Laboratory strain related to strain 8325-429
    SH10008325-4 with rsbU restored15
    ALC6374RN6390 ΔsarZThis work
    ALC6366SH1000 ΔsarZThis work
    ALC6367RN6390 ΔsarZ/sarZ complementThis work
    ALC6368RN6390 ΔsarZ with pEPSA5This work
    ALC6369RN6390 ΔsarZ with pEPSA5::sspAThis work
    ALC6370RN6390 ΔsarZ with pEPSA5::mgrAThis work
    ALC6410RN6390 ΔsarZ ΔsarAThis work
    ALC6409RN6390 ΔsarZ with pRN6735 (blaZ promoter driving RNAIII)This work
E. coli strain TOP10General cloning strain
Plasmids
    pMADE. coli-S. aureus shuttle vector containing a thermosensitve origin of replication, bgaB, Emr Apr1
    pEPSA5E. coli-S. aureus shuttle vector containing a xylose-inducible promoter, Apr Cmr13
    pALC1484E. coli-S. aureus shuttle vector derived from pSK236 containing the gfpuvr gene, Apr Cmr17
    pALC1540sarA triple promoter driving the expression of gfpuvr in pALC148421
    pALC1435sarA P1 promoter driving the expression of gfpuvr in pALC148421
    pALC1743agr P3 promoter driving the expression of gfpuvr in pALC148417
    pALC1741spa promoter driving the expression of gfpuvr in pALC148419
    pALC1740hla promoter driving the expression of gfpuvr in pALC148419
    pALC2831sspA promoter driving the expression of gfpuvr in pALC1484This work
    pALC2566mgrA promoter driving the expression of gfpuvr in pALC1484This work
    pALC5448pMAD containing a sarA deletion fragment generated by PCR34
    pRN6735blaZ promoter driving the expression of promoterless RNAIII, Cmr35