TABLE 4

Quantitative RT-PCR analysis of virulence-associated gene transcripts

SPy no.DesignationbQuantity of transcripta (mean ± SEM)rgg/wild-type ratioc
Wild-type NZ131speB mutant NZ131rgg mutant NZ131
0167 d slo (SLO) 0.09 ± 0.0 0.10 ± 0.0 1.25 ± 0.1 13.9
0212 speG (SpeG)0.42 ± 0.10.56 ± 0.00.33 ± 0.00.8
0747 None (none) 0.31 ± 0.0 0.26 ± 0.0 1.00 ± 0.1 3.2
0861 mac (Mac) 0.03 ± 0.0 0.04 ± 0.0 0.06 ± 0.0 2.0
1008 speH (SpeH) 0.090 ± 0.0 0.11 ± 0.1 0.18 ± 0.1 2.0
1357 grab (Grab) 1.80 ± 0.2 1.71 ± 0.3 0.20 ± 0.1 0.1
1436 mf-3 (MF-3) 0.14 ± 0.0 0.14 ± 0.0 3.47 ± 0.0 24.8
1801 isp-2 (Isp-2)3.91 ± 0.93.36 ± 0.93.63 ± 0.80.9
2025 isp-1 (Isp-1) 1.07 ± 0.3 0.63 ± 0.1 0.46 ± 0.1 0.4
  • a The gene-specific cDNA values were normalized to the quantity of gyrA cDNA in each sample. The mean ± the standard error of the mean of two independently isolated RNA samples is given.

  • b Gene designations are in italics, and the corresponding protein designations are in parentheses.

  • c Quantity of gyrA-normalized cDNA in rgg mutant NZ131 ÷ quantity in wild-type NZ131.

  • d Boldface denotes differences in transcripts between wild-type NZ131 and rgg mutant NZ131 of less than 0.5 or greater than 1.5.