TABLE 2.

Collagen and fibronectin binding in sar and agr mutants

Parameter and strainBinding in:
Wild typea (dpm)Mutantb (% binding)
sarsar (pSARA)agrsar agrsar agr (pSARA)
CBC
    UAMS-1197,544 ± 36,722217 ± 14c (273 ± 20)99 ± 3117 ± 18c (97 ± 10)258 ± 15c (255 ± 20)1.12 ± 0.03
    UAMS-601133,493 ± 22,115235 ± 14c (241 ± 24)118 ± 5118 ± 18c (97 ± 1)2.58 ± 15c (232 ± 9)1.45 ± 0.03
FBC
    UAMS-11,274,323 ± 237,29732 ± 9c (52 ± 3)99 ± 17116 ± 20c (82 ± 2)38 ± 14c (52 ± 3)0.95 ± 0.11
    UAMS-601817,297 ± 140,13038 ± 8c (66 ± 3)53 ± 8c93 ± 18 (107 ± 8)23 ± 6c (95 ± 10)0.60 ± 0.12c
    RN6390370,375 ± 77,71574 ± 14c101 ± 9347 ± 68c151 ± 372.36 ± 0.19
    DB1,481,309 ± 188,83634 ± 11cND106 ± 3442 ± 15cND
    SC-11,410,816 ± 66,68230 ± 5c101 ± 5108 ± 869 ± 6c1.23 ± 0.06
    S6C2,589,910 ± 56,62548 ± 5cND110 ± 876 ± 9ND
    Newman295,398 ± 73,21255 ± 2cND125 ± 1575 ± 10ND
  • a Values for each wild-type strain represent the numbers of counts bound. Results are means ± standard errors of the means and represent the averages of six assays, each of which was done in duplicate.

  • b Values for sarA, agr, and sarA agr mutants are reported as the percent binding relative to the corresponding wild-type strain. Values in parentheses show the percent change in transcription relative to that of the corresponding parent strain based on densitometric analysis of Northern slot blots. Results are means ± standard errors of the means and represent the averages of two Northern blots.

  • c Statistical significance (P < 0.05) as determined by Student’s paired t test to compare each mutant to the corresponding parent strain. In the case of the pSARA-complemented sarA agr mutant, statistical significance was assessed based on comparison to the corresponding agr mutant. ND, not determined.