TABLE 2

Resistance of the nonphagocytosed H. ducreyi population to repeated phagocytosis by granulocytes, as measured by FACS

Bacterial population and incubationa% Phagocytosisb after the following min:
103060
H. ducreyi
First28 ± 0.7 (26.8 ± 4.9)36 ± 1.4 (40 ± 5.6)44 ± 0.7 (53 ± 1.4)
Second (supernatant of the first incubation)4 ± 5.0 (5 ± 1.4)3 ± 2.1 (7 ± 2.8)2 ± 1.4 (3 ± 2.2)
E. coli (control)
First90 ± 1.391 ± 3.093 ± 2.1
Second (supernatant of the first incubation)67 ± 0.841 ± 7.038 ± 1.5
  • a See Materials and Methods for bacterial concentration and procedures for the phagocytosis of bacteria.

  • b Results are reported as the mean and SD for three experiments. For H. ducreyi, values are for heat-killed (live) bacteria tested with antiserum to LOS.