TABLE 1.

ivi genes identified by using pPGIVET

ivi genesORF(s) and possible promoter(s)aBLAST search resultb
No. of ORF(s)Size (bp)RegionSequence
ivi11483−35TTCATA(CCGACA)No homology
−10TATA
ivi22294, 150−35TTGCCCNo homology; I = 99%
−10TAAA
ivi311,944−35TTGTACNH(3)-dependent NAD(+) synthetase (nadE); I = 39%
−10TAATTT
ivi41806−35TTGTCTNo homology
−10ATTATT
ivi51902−35TTGAAAIS195, insertion sequence-like element associated with protease genes (Porphyromonas gingivalis); I = 99%
−10AATAA
ivi61474−35TTGTATAntisense carbamoyl phosphate synthetase (Bacillus subtilis)
−10TAA
ivi71975−35TTGTCCNo homology; clustered with immunoreactive 63-kDa antigen PG102 and immunoreactive 46-Da antigen PG99 (Porphyromonas gingivalis)
−10TAAAA
ivi811,464−35TTGACGNo homology
−10AAATA
ivi91735−35GTGACANo homology; upstream of RecA-like protein
−10TTTAA(TTG start)
ivi1012,274−35TTGCATPutative TonB-dependent outer membrane receptor protein (Bacteroides fragilis); I = 33%
−10TATCAT
ivi111882−35TGCTCA(TTCAAC)Immunoreactive 33-kDa antigen PG125c (Porphyromonas gingivalis); I = 99%
−10TAAT(TTAATT)
ivi121212−35AGGACAAntisense phosphoribosylglycinamide formyltransferase (Bacillus subtilis); I = 37%
−10TATAAT
ivi131678−35TTCACANo homology; adjacent to phenylalanyl tRNA synthetase
−10TAGTA(TTG start)
ivi1412,037−35TTGAAAUvrB (Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, etc.); I = 56%
−10TAATTA
  • a The table presents the potential −10 region and −35 region best-matching E. coli promoter consensus sequences: TATAAT for the −10 region and TTGACA for the −35 region.

  • b I, identity.

  • c B. Ross et al. (41a).