TABLE 1

Translocation and tyrosine phosphorylation of CagA, induction of the scattering phenotype, and secretion of IL-8 in AGS cells infected with H. pyloria

Bacterial strainMutated ORFbCagA expres- sionCagA trans- location/phos- phorylationInduction of scattering phenotypeInduction of IL-8 secretion
P1 wt++++
P12 wt++++
G27 wt++++
J99 wt++
P12ΔvacAHP0887++++
P1ΔalpA/BHP0912/HP0913+
P12Δcag PA1HP520-HP547
P1ΔvirB4HP0544+
P1ΔvirB7HP0532+
P1ΔvirB8HP0529+
P1ΔvirB9HP0528+
P1ΔvirB10HP0527+
P1ΔvirB11HP0525+
P1ΔvirD4HP0524++
P1ΔcagAHP0547+
G27ΔcagFHP0543+
P1ΔcagHHP0541+
G27ΔcagIHP0540+
G27ΔcagMHP0537+
P1ΔcagA/cagAHP0547++++
P1ΔvirD4/virD4HP0524++++
  • a All H. pylori strains are type I isolates. cag PA1 mutagenesis and genetic complementation are described in Materials and Methods (see also Fig. 1). Bacterial infection was for 6h at an MOI of 100. CagA translation and tyrosine phosphorylation were detected by immunoblot analysis. IL-8 production was determined by ELISA. wt, wild type

  • b From Tomb et al. (39).