TABLE 2.

Spirochetal load determined by quantitative TaqMan RT-PCR for Borrelia 16S rRNA in biceps muscle from NHPs inoculated with borreliaea

NHP no.StrainInoculationResults obtained in the absence or presence of host nucleic acid added to the standard-curve sample used for Borrelia measurementsb
AbsencePresence
Input μg of RNALoadSample 1Sample 2
Input μg of RNALoadInput μg of RNALoad
099N40Needle1.20.00021010.1500
1773.80.00011010.0300
1992.70.010110.003710.0300
3833.40.01131010.0100
154N40Tick bite2.10.001610.001110.4000
1922.60.004310.001911.6000
2112.70.00481010.0200
24220.00071010
105PbiNeedleNot done10.002010.0300
321Not done10.001110.0200
012Not done0.50
981Not done0.50
  • a NHPs were inoculated with B. burgdorferi sensu stricto strain N40 or B. garinii strain Pbi. Load is reported as number of spirochetes per microgram of skeletal muscle RNA.

  • b One microgram of host nucleic acid was added to the standard-curve sample to control for the possibility of PCR inhibition by host nucleic acid. In the presence of host nucleic acid, a different areas of the biceps muscle (samples 1 and 2) were examined to search for variations in spirochetal loads between different areas of the same muscle.