TABLE 2.

Individual guinea pig anti-CS6 antibody-secreting cell and IgG antibodies induced by topical immunizationa

Immunization groupAnimalNo. of antibody-secreting cells per 106 cells
IgGIgAIgM
PBS1112
2204
CS6-LT18112
24231
33114
46357
  • a Guinea pigs were immunized on the skin with LT (50 μg) and rCS6 (100 μg) at zero time and after 4 and 8 weeks. Seven days after the last immunization, spleen and lymph node cell suspensions were subjected to an ELISPOT assay to detect the number of anti-CS6 antibody-secreting cells induced by the topical immunization. The levels of IgG antibodies to CS6 were measured 7 days after the last immunization.