TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristic(s)aSource or reference
Strains
    E. coli
        XL2-BluerecA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10(Tetr) amy Cmr]Stratagene
        M15Nals Strs Rifs Thi Lac Ara+ Gal+ Mtl F RecA+ Uvr+ Lon+Qiagen
    S. suis
        10Virulent serotype 2 strain29
        10ΔFBPSIsogenic fbps mutant of strain 10This work
Plasmids
        pGEM7Zf(+)Replication functions pUC, AmprPromega Corp.
        pKUN19Replication functions pUC, Ampr14
        pIC19RReplication functions pUC, Ampr16
        pDL282Replication functions of pBR322 and pVT736-1, Ampr Spcr25
        pIC-spcpIC19R containing Spcr gene of pDL282Laboratory collection
        pQE-30Replication functions pBR322, Ampr, expression vector, six-His tagQiagen
        pQE-30-FBPSpQE-30 containing the 1.8-kb fbps geneThis work
        pREP4Replication functions pACYC, Kanr, lacI geneQiagen
        pE194Emr11
        pIVS-EReplication functions of pWV01, Spcr, promoterless erm gene of pE19420
        pIVS-31pIVS-E containing 200 bp showing homology to Streptococcus gordonii flpa20
        pFBPS7-46pGEM7Zf(+) containing EcoRI-EcoRI fragment of fbpsThis work
        pFBPS7-47pFBPS7-46 in which 382-bp SalI-SalI fragment is replaced by 1.2-kb Spcr from pIC-spcThis work
  • a Tetr, tetracycline resistant; Cmr, chloramphenicol resistant; Ampr, ampicillin resistant; Spcr, spectinomycin resistant; Kanr, kanamycin resistant.