TABLE 1.

Oligonucleotides used in this study

DesignationSequencea
ProsseA-For-HindIII5′-AGGAAGCTTAAGAAGAGAACAACGGCAAG-3′
ProsseA-Rev-EcoRI5′-CACGAATTCACGATAGATAATTAACGTGC-3′
SseB-Rev-EcoRV5′-AGTGATATCTGAGTACGTTTTCTGCGC-3′
SscB-For-EcoRI5′-CGGAATTCAATAGGTATGATGATGAAAG-3′
SseF-Rev-EcoRV5′-ACGATATCTCCCCGAGATGTATGATCAG-3′
SseG-Rev-EcoRV5′-ACGGATATCCGGCGCACGTTGTTCTGGCG-3′
SifA-Pro-For-EcoRI5′-CCGGAATTCGTGASTATAAGCGATTAATTGC-3′
SifA-For-EcoRI5′-CCGGAATTCATTTTTACTCCAGTATAAG-3′
SifB-Pro-For-EcoRI5′-CCGGAATTCTGCCCTACCGCTAAAC-3′
SifB-Rev-EcoRV5′-ACGGATATCACTCTGGTGATGAGCCTC-3′
SseJ-Pro-For-EcoRI5′-CCGGAATTCACATAAAACACTAGCAC-3′
SseJ-Rev-EcoRV5′-ACGGATATCTTCAGTGGAATAATGATGAGC-3′
  • a Restriction sites used for cloning of PCR products are underlined.