TABLE 1.

Cytokine levels in lung homogenates of mice inoculated with LPS, PLC, or ETAa

CytokineInoculumMean ± SEM of cytokine level (pg/ml) at
Control26122448
TNF-αLPS<15517.7 ± 94.7***171.7 ± 12.3*146.1 ± 23.6*32.2 ± 17.1<15
PLC<1593.7 ± 4.6***41.5 ± 20.8**<15<15<15
ETA<15<15<15<15<15<15
IL-1βLPS<10670.7 ± 70.9***678.9 ± 173.5***710.0 ± 133.0***631.0 ± 125.2**240.0 ± 53.5
PLC<10458.8 ± 27.8***744.5 ± 55.5***237.0 ± 2.9*90.3 ± 3.3<10
ETA<10<10<1018.6 ± 6.7*17.5 ± 1.9*11.3 ± 1.4
IL-6LPS<204,408 ± 1899*6,234 ± 1187**4,702 ± 1087*768 ± 294.4*5,367 ± 976.0*
PLC<207,588 ± 1826*6,265 ± 1346**1,015 ± 658.2*2,413 ± 1317*<20
ETA<20<2083.3 ± 68.0503.5 ± 396.3*<20<20
IL-18LPS741 ± 271714 ± 276797 ± 185775 ± 86.8937 ± 67618 ± 51.9
PLC700 ± 54.2494 ± 51.6509 ± 26.0564 ± 59.2510 ± 52.8594 ± 113.9
ETA825 ± 80.1660 ± 50.3*355 ± 60.7***335 ± 22.3***210 ± 12.6***125 ± 5.9***
IFN-γLPS55.9 ± 2.135.3 ± 2.951.5 ± 4.663.0 ± 4.8266.1 ± 72.9**244.9 ± 11.1**
PLC44.4 ± 7.442.8 ± 3.553.7 ± 7.268.2 ± 4.756.4 ± 6.381.6 ± 9.3
ETA74.4 ± 13.358.6 ± 18.876.4 ± 16.263.6 ± 4.690.9 ± 36.275.2 ± 8.3
MIP-1αLPS<15349.0 ± 67.7***400.3 ± 56.4***284.1 ± 23.0***235.8 ± 27.0***189.6 ± 19.5**
PLC<15275.1 ± 27.8***259.1 ± 55.5***30.0 ± 2.918.8 ± 3.3<15
ETA<15<15<1519.0 ± 6.7*16.8 ± 3.2*<15
MIP-2LPS<153,543 ± 1194***1,657 ± 166*1,169 ± 89.8*870.5 ± 116.2*541.6 ± 20.9
PLC<151,875 ± 77.5***1,014 ± 272.4***400.5 ± 38.4270.1 ± 57.2215.0 ± 35.9
ETA<15<15<15163.3 ± 28.5*172.3 ± 11.1***145.1 ± 11.6***
  • a Groups of three to five mice were inoculated with either LPS (10 μg/mouse), PLC (0.5 μg/mouse), or ETA (2μg/mouse). Control mice received vehicle. Lungs were removed at the indicated time points (in hours) and cytokine levels were measured in lung homogenates.

  • b *, P < 0.05; **, P < 0.01; ***, P < 0.001 versus respective control by factorial ANOVA.