TABLE 1.

Serum CS6 and LT IgG levels in guinea pigs immunized on the skin with CS6 and LT IgG

AdjuvantAntigenAntibodyMean serum IgG level (ELISA units)
Prebleed3 wk6 wk8 wk
LT (100 μg)CS6 (200 μg)Anti-CS6 IgG30 (7)b55 (7)1,588 (5)4,383 (3)
Anti-LT IgG57 (7)104 (7)1,258 (5)3,764 (3)
LT (50 μg)CS6 (100 μg)Anti-CS6 IgG23 (10)55 (10)1,155 (8)12,471 (1)
Anti-LT IgG52 (10)87 (10)297 (8)4,933 (1)
LT (25 μg)CS6 (50 μg)Anti-CS6 IgG24 (6)26 (6)103 (4)1,084 (2)
Anti-LT IgG43 (6)77 (6)106 (4)381 (2)
LT (12 μg)CS6 (25 μg)Anti-CS6 IgG13 (6)26 (6)68 (4)243 (2)
Anti-LT IgG35 (6)65 (6)112 (4)153 (2)
PBSAnti-CS6 IgG18 (6)27 (6)54 (5)36 (2)
Anti-LT IgG42 (6)73 (6)113 (5)95 (2)
  • a Guinea pigs were immunized on the skin with LT and CS6 at zero time and after 3 and 6 weeks. A control group was given the same volume of PBS on the skin. Serum was collected the day before immunization and 2 weeks after the last immunization. CS6- and LT-specific IgG titers were determined by ELISA. Levels in serum are expressed in ELISA units (the inverse of the titer at which the optical density at 405 nm was equal to 1.0).

  • b The numbers in parentheses are numbers of animals.