TABLE 1.

Cytokine elaboration from human vaginal and endo- and ectocervical EC following exposure to purified rMG309ca

CytokineMean cytokine concn (pg/ml) from:
Vaginal ECEctocervical ECEndocervical EC
rMG309cPBSrMG309cPBSrMG309cPBS
IL-634.8 ± 5.127 ± 2.627.1 ± 3.214.2 ± 0.63114 ± 3.1*26.6 ± 2.9
IL-8186 ± 0.6*132 ± 111,792 ± 38*167 ± 1762,997 ± 6,667*3,705 ± 1,440
G-CSF17 ± 0.512 ± 0.1368 ± 7.5*181 ± 3.2200 ± 5.1*24 ± 2.4
GM-CSF<LL<LL10.8 ± 0.9*4.9 ± 0.432 ± 4.0*6 ± 1.9
  • a Vaginal EC and ecto- and endocervical EC were stimulated with rMG309c (0.5 μg/well) or an equal volume of the PBS vehicle as a control. Culture supernatants were collected 6 h after stimulation to quantify secreted cytokines as described in Materials and Methods. Values are expressed as means ± standard errors of the means. <LL (less than the lower limit) indicates that values were too low to be calculated from the standard curve. *, significantly increased compared to PBS vehicle control (P < 0.05; Student's t test).