TABLE 1.

Oligonucleotides used to determine plasmid contents

PlasmidPrimerPrimer coordinatesCorresponding gene (coordinates)
lp54BBA60-5′40981-40957BBA60, surface lipoprotein P27 (40981-40151)
BBA60-3′40151-40175
cp26BBB03-5′2186-2162BBB03, hypothetical protein (2186-840)
BBB03-3′840-864
cp9BBC10-5′6808-6784BBC10, Rev protein (rev) (6808-6284)
BBC10-3′6284-6308
1p17BBD11-5′6681-6705BBD11, conserved hypothetical protein (6681-7631)
BBD11-3′7631-7607
1p25BBE17-5′10709-10685BBE17, hypothetical protein (10709-10203)
BBE17-3′10203-10227
lp28-1BBF18-5′9561-9585BBF18, putative transposase-like protein, authentic frameshift (9561-10049)
BBF18-3′10049-10025
lp28-2BBG13-5′12355-12331BBG13, hypothetical protein (12355-11504)
BBG13-3′11504-11528
lp28-3BBH18-5′12105-12129BBH18, hypothetical protein (12105-13217)
BBH18-3′13217-13193
lp28-4BBI28-5′17874-17850BBI28, hypothetical protein (17874-17305)
BBI28-3′17305-17329
lp38BBJ23-5′16505-16529BBJ23, hypothetical protein (16505-17326)
BBJ23-3′17326-17302
lp36BBK23-5′15760-15736BBK23, hypothetical protein (15760-14837)
BBK23-3′14837-14861
cp32-8BBL39-5′26370-26394BBL39, ErpA protein (26370-26900)
BBL39-3′26900-26876
BBL40-5′26931-26955BBL40, ErpB2 protein (26931-28064)
BBL40-3′28064-28040
cp32-6BBM32-5′20732-20756BBM32, plasmid partition protein, putative (20721-21467)
BBM32-3′21417-21393
BBM38-5′26380-26404BBM38, ErpK protein (26245-27012)
BBM38-3′26887-26863
cp32-9BBN28-5′17302-17326BBN28, lipoprotein (17302-17727)
BBN28-3′17596-17572
BBN32-5′20828-20852BBN32, plasmid partition protein, putative (20817-21569)
BBN32-3′21449-21425
cp32-7BBO32-5′20777-20801BBO32, plasmid partition protein, putative (20766-21512)
BBO32-3′21382-21358
BBO40-5′27123-27147BBO40, ErpM protein (26893-27981)
BBO40-3′27615-27591
cp32-1BBP32-5′20777-20801BBP32, plasmid partition protein, putative (20766-21503)
BBP32-3′21440-21416
BBP38-5′26235-26259BBP38, ErpA protein (26247-26765)
BBP38-3′26765-26741
lp56BBQ05-5′2744-2768BBQ05, antigen, P35, putative (2744-3493)
BBQ05-3′3493-3469
BBQ08-5′5814-5790BBQ08, plasmid partition protein, putative (5830-5072)
BBQ08-3′5185-5209
cp32-4BBR41-5′26109-26133BBR41, conserved hypothetical protein (26077-26817)
BBR41-3′26707-26683
BBR42-5′26922-26946BBR42, outer surface protein F (26853-27524)
BBR42-3′27370-27346
cp32-3BBS35-5′21268-21292BBS35, plasmid partition protein, putative (21257-21994)
BBS35-3′21806-21782
BBS41-5′26743-26767BBS41, outer surface protein G (26708-27295)
BBS41-3′27199-27175
lp5BBT03-5′1208-1232BBT03, hypothetical protein (1208-1573)
BBT03-3′1573-1549
lp21BBU04-5′1493-1517BBU04, conserved hypothetical protein (1486-2625)
BBU04-3′2578-2554