TABLE 1.

Strains and plasmids

Strain or plasmidDescription or genotypeResistance marker(s)Source or reference
Strains
    E. coli strains
        DH5α-ECloning strainNoneInvitrogen
        BW25141Cloning strainNone20
    S. epidermidis ATCC R97-03
PIA control strainNone65
    S. aureus strains
        LACCA-MRSA USA300-0114Erm, Tet72
        MN8Toxic shock isolateNone50
        MN8mPIA overproducerNone50
        MN8icaΔica::tetTet50
        RN4220Restriction modification deficientNone45
        SH1000sigB+ derivative of NCTC8325-4None33
        SH1001SH1000 Δagr::tetMTet33
        SH1002SH1000 sarA::kanKan33
        AH528SH1000 rsbU::Tn5KanThis work
        AH595SH1000 Δica::tetTet11
        AH750SH1000 Δaur Δspl::ErmErm11
        AH1012SH1000 ΔsigBNoneThis work
        AH1096LAC ΔsigBErmThis work
        AH1106SH1000 ΔsigB Δagr::tetMTetThis work
        AH1136SH1000 ΔsigB Δaur Δspl::ErmErmThis work
        AH1203LAC Δagr::tetMErm, TetThis work
        AH1204LAC ΔsigB Δagr::tetMErm, TetThis work
        AH1308LAC Δica::tetTetThis work
Plasmids
    pAH1P3agr-mCherryCamThis work
    pAH6Pasp23-mCherryCamThis work
    pAH8P3agr-mCherryErmThis work
    pAH12Pasp23-mCherryErmThis work
    pALC2073Shuttle vector with tet promoterCam4
    pALC2084Tet-inducible GFPCam4
    pALC2109sigB gene cloned into pALC2073Cam4
    pBursaMariner hopping plasmidCam, Erm2
    pDB59P3agr-YFPCam75
    pFA545Mariner transposaseTet2
    pKOR1Gene replacement plasmidCam3
    pTN11Tn5 hopping plasmidCam, KanThis work