TABLE 5.

A. pleuropneumoniae genes which are downregulated during adherence to SJPL cellsa

Locus tagGeneDescriptionFold change
Hypothetical/unclassified/unknown
    APL_1887Esterase domain-containing protein−6.273
    APL_1284Putative DNA-binding protein−3.076
    APL_0049Hypothetical protein−2.982
    APL_0970Hypothetical protein−2.980
    APL_1100Hypothetical protein−2.928
    APL_1437Hypothetical protein−2.751
    APL_0116Hypothetical protein−2.566
    APL_0389ompP4Lipoprotein E precursor, predicted secreted acid phosphatase−2.509
    APL_0704Potential type III restriction enzyme−2.293
    APL_1365HlyHypothetical protein−2.174
    APL_0110Hypothetical protein−2.144
    APL_1396Hypothetical protein−2.125
    APL_0756Hypothetical protein−2.098
    APL_0889Hypothetical protein−1.757
Energy metabolism
    APL_0434gapAGlyceraldehyde-3-phosphate dehydrogenase−4.981
    APL_0892fdxGFormate dehydrogenase, nitrate-inducible, major subunit−4.853
    APL_0771lpdADihydrolipoyl dehydrogenase−4.765
    APL_1379ccpCytochrome c peroxidase−4.447
    APL_0983tktATransketolase 2−3.790
    APL_0894fdxHFormate dehydrogenase, iron-sulfur subunit−3.627
    APL_1251pgk3-Phosphoglycerate kinase−3.625
    APL_1450fbpFructose-1,6-bisphosphatase−3.315
    APL_0181gloALactoylglutathione lyase−3.046
    APL_1925tpiATriosephosphate isomerase−3.023
    APL_1091aspAAspartate ammonia-lyase−2.722
    APL_0688torZTrimethylamine-N-oxide reductase precursor−2.687
    APL_1011adh2Aldehyde-alcohol dehydrogenase 2−2.642
    APL_0772aceFDihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (E2)−2.620
    APL_1137pgiGlucose-6-phosphate isomerase−2.595
    APL_0486maeANADP-dependent malic enzyme−2.416
    APL_1526frdDFumarate reductase subunit D−2.267
    APL_0101nrfBNitrate reductase, cytochrome-c-type protein−2.231
    APL_1250fbaFructose bisphosphate aldolase−2.124
Transport and binding proteins: others
    APL_1620cbiOPredicted ABC-type cobalt transport, ATPase component−2.108
    APL_0447lctPPutative l-lactate permease−2.068
    APL_0719Putative phosphate permeases−1.816
    APL_0791Transmembrane transport protein-permease−1.702
Regulatory functions
    APL_0656hlyXRegulatory protein HlyX−2.727
    APL_0629cpxRTranscriptional regulatory protein CpxR−2.367
Purines, pyrimidines, nucleosides, and nucleotides
    APL_0769ushAUshA precursor−3.470
    APL_0646cpdB2′,3′-Cyclic-nucleotide 2′-phosphodiesterase precursor−2.778
    APL_1014deoDPurine-nucleoside phosphorylase−2.247
Protein fate
    APL_1154Putative zinc protease−2.401
    APL_1456slyDFkbP-type peptidyl-prolyl cis-trans isomerase−2.313
Protein synthesis
    APL_0740rpsA30s ribosomal protein S1−1.608
Cellular processes
    APL_1445apxICRTX-I toxin-activating lysine-acyltransferase ApxIC−4.117
    APL_0956apxIIARTX-II toxin determinant A−4.052
    APL_0004sodCSuperoxide dismutase (Cu/Zn) precursor−3.193
Cell envelope
    APL_1494ftpACOG0783: DNA-binding ferritin-like protein (oxidative damage protectant)−4.223
    APL_0652manBPhosphomannomutase−3.375
Central intermediary metabolism
    APL_0645ackAAcetate kinase−3.190
    APL_1899ppaInorganic pyrophosphatase−2.207
  • a Fifty-two genes.