TABLE 1.

Cytokine levels in spleen cell supernatants from mice immunized via different routes

Vaccination groupCytokine concn (pg/ml)a
IFN-γIL-12IL-4IL-10TNF-αTGF-β
Groups after immunization
    Control21 ± 322 ± 315 ± 216 ± 411 ± 193 ± 16
    Liposomes i.p.22 ± 324 ± 314 ± 220 ± 113 ± 5105 ± 6
    Liposomes s.c.23 ± 422 ± 213 ± 318 ± 412 ± 2102 ± 8
    Liposomal LAg i.p.120 ± 7b95 ± 8b48 ± 5b22 ± 419 ± 5114 ± 6
    Liposomal LAg s.c.28 ± 325 ± 321 ± 331 ± 314 ± 4924 ± 87b
Groups 4 mo after infection
    Control31 ± 227 ± 493 ± 8230 ± 2124 ± 41,652 ± 81
    Liposomes i.p.37 ± 228 ± 387 ± 8228 ± 1424 ± 31,624 ± 113
    Liposomes s.c.32 ± 430 ± 486 ± 6220 ± 1129 ± 41,540 ± 125
    Liposomal LAg i.p.274 ± 18b168 ± 10b37 ± 4b33 ± 4b126 ± 9b113 ± 9b
    Liposomal LAg s.c.38 ± 332 ± 397 ± 7228 ± 828 ± 41,680 ± 77
  • a Data are for five mice and are expressed as means ± standard errors. Data are representative of two independent experiments with similar results.

  • b P < 0.001 in comparison to control groups.