TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristic(s)Reference or source
E. coli strains
    CY70E128012 ΔpstCA::kan KanrThis study
    CY88E128012 ΔpstCAThis study
    CY91E128012 ΔpstCA phoBThis study
    CY95E128012 ΔphoB::kan KanrThis study
    E128012Wild-type atypical EPEC O114:H2 25
    E1E128012 TnphoA mutant, pstS::TnphoA KanrThis study
    E2E128012 TnphoA mutant, pstS::TnphoA KanrThis study
    E3E128012 TnphoA mutant, KanrThis study
    E4E128012 TnphoA mutant, pstA::TnphoA KanrThis study
    E5E128012 TnphoA mutant, pstA::TnphoA KanrThis study
    E6E128012 TnphoA mutant, pstC::TnphoA KanrThis study
C. rodentium strains
    ICC169Derivative of C. rodentium ICC168, Nalr 27
    ICA15ICC169 ΔpstCA::kan Kanr NalrThis study
    ICA18ICC169 ΔpstCA ΔphoB::kan Kanr NalrThis study
Plasmids
    pBlueScript (pBSII) SKHigh-copy-no. cloning vector; AmprAgilent Technologies
    pGEM-T EasyHigh-copy-no. cloning vector; AmprPromega
    pACYC184Medium-copy-no. cloning vector; Camr TetrNew England Biolabs
    pRT733Delivery vector of TnphoA, derivative of suicide vector pJM703.1; Ampr Kanr 37
    pKD4FRT-flanked Kanr cassette template 12
    pACBSRI-SceI and λ Red recombinase expression plasmid with arabinose-inducible expression; Camr 18
    pCP20FLP helper plasmid, temp-sensitive replication; Ampr Camr 10
    pAC11.8-kb PCR product containing wild-type E. coli E128012 pstCA cloned into SmaI site of pBSII; AmprThis study
    pAC21.9-kb BamHI/EcoRV fragment from pCA1 containing wild-type E. coli E128012 pstCA cloned into the BamHI/EcoRV sites of pACYC184; CamrThis study
    pAC41.9-kb fragment containing wild-type C. rodentium ICC169 pstCA cloned into the EcoRV/SalI sites of pACYC184; CamrThis study