TABLE 1.

Analysis of molecular masses of CssA and CssB

SubunitLength (amino acids) of mature proteinMolecular mass, kDa (mean ± SEM)
PredictedaSDS-PAGEbESI-MSc
CssA13614.9617.39 ± 0.1918.46 ± 0.18d, 15.06 ± 0.17e
CssB14616.0616.20 ± 0.1815.92 ± 0.2
  • a Calculated based on the gene sequence by the Expasy proteomic server (http://www.expasy.ch/).

  • b Calculated with respect to standard marker proteins in a gel documentation system from six different SDS-PAGEs.

  • c Analyzed with the API 2000.

  • d Mass of native form of CssA.

  • e Mass obtained after in-source fragmentation.