TABLE 1.

Oligonucleotide primers used to amplify virulence-associated genes

Primer designationPrimer sequenceTargetSize of PCR product (bp)Reference
chuA.15′-GACGAACCAACGGTCAGGAT-3′ chuA 279(9)
chuA.25′-TGCCGCCAGTACCAAAGACA-3′(9)
yjaA.15′-TGAAGTGTCAGGAGACGCTG-3′ yjaA 211(9)
yjaA.25′-ATGGAGAATGCGTTCCTCAAC-3′(9)
TspE4C2.15′-GAGTAATGTCGGGGCATTCA-3′TspE4.C2152(9)
TspE4C2.25′-CGCGCCAACAAAGTATTACG-3′(9)
papC.15′-GACGGCTGTACTGCAGGGTGTGGCG-3′ papC 328(33)
papC.25′-ATATCCTTTCTGCAGGGATGCAATA-3′(33)
papG.I.15′-TCGTGCTCAGGTCCGGAATTT-3′ papGI 461(23)
papG.I.25′-TGGCATCCCCCAACATTATCG-3′(23)
papG.II.15′-GGGATGAGCGGGCCTTTGAT-3′ papGII 190(23)
papG.II.25′-CGGGCCCCCAAGTAACTCG-3′(23)
papG.III.15′-GGCCTGCAATGGATTTACCTGG-3′ papGIII 258(23)
papG.III.25′-CCACCAAATGACCATGCCAGAC-3′(23)
papG.II/III.15′-GACTCTTTCTGTGTCTTGCG-3′ papGII + papGIII254This study
papG.II/III.25′-GAACCAGATAGTACTCCTGG-3′This study
sfa.15′-CTCCGGAGAACTGGGTGCATCTTAC-3′ sfa/foc 410(33)
sfa.25′-CGGAGGAGTAATTACAAACCTGGCA-3′(33)
hly.15′-AGGTTCTTGGGCATGTATCCT-3′ hlyC 556(5)
hly.25′-TTGCTTTGCAGACTGCAGTGT-3′(5)
cnf1.15′-CAGTGACCGGATCTCCGTTAT-3′ cnf-1 230(38)
cnf1.25′-CGTGTAATTCTTCTGTACTTCC-3′(38)
aer.15′-AAACCTGGTTTACGCAACTGT-3′ aer (iucC)269(5)
aer.25′-ACCCGTCTGCAAATCATGGAT-3′(5)
fyuA.15′-TGATTAACCCCGCGACGGGAA-3′ fyuA 780(31)
fyuA.25′-CGCAGTAGGCACGATGTTGTA-3′(44)
irp2.15′-AAGGATTCGCTGTTACCGGAC-3′ irp-2 280(44)
irp2.25′-TCGTCGGGCAGCGTTTCTTCT-3′(44)
hra.15′-CAGAAAACAACCGGTATCAG-3′ hra 260This study
hra.25′-ACCAAGCATGATGTCATGAC-3′This study
pheR.15′-GCCGCAATCTTAAGCAGTTG-3′ pheR 350This study
pheR.25′-GCACGACATTTCACGTCAGT-3′This study
yjgB.15′-ACCTTGCTCGCAGTTGATCT-3′ leuX (yjgB) This study