TABLE 1.

Oligonucleotide primers and LightCycler hybridization probes

Target geneGenBank accession no.PrimerSequencea
gyrBD10489gyrUTTATGGTGCTGGGCAAATACA
gyrLCACCATGTAAACCACCAGATA
gyrFL1ATTTTAACTGTTTTACATGCTGGTGGTAA-F
gyrLC1Red640-TTTGGCGGTGGCGGATACA-ph
clfAZ18852clfUGGCGTGGCTTCAGTGCTTGTA
clfLCACCAGTTACCGGCGTTTCTTC
clfFL1GGCGCAAAATCCAGCACAACAGGAA-F
clfLC1Red640-CGACACAATCATCATCAACAAATGC AAC-ph
  • a F, fluorescein; Red640, LightCycler-Red 640-N-hydroxysuccinimide ester; ph, 3′-phosphate.