TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotypeRelevant propertiesSource or reference
S. aureus strains
    RN4220Restriction-deficient 8325-4 mutant22
    NewmanExpresses clumping factor8
    DU5944Newman clfA2::Tn917 Emr clfB::TcrDeficient in clumping factors A and B29
    P1Expresses FnBPs37
    P1 FnBPP1 fnbA::Tcr fnbB::EmrDeficient in FnBPA and -BT. J. Foster
    V329Expresses Bap7
    m556V329 bap::Tn917 EmrDeficient in Bap7
    SA113Expresses PIA/PNSG6
    SA113ΔicaSA113 ΔicaADBC::TcrDeficient in PIA/PNSG6
Plasmids
    pBT2Shuttle plasmid3
    pBT2:BappBT2 with bap gene7