TABLE 1.

FCR3 VAR2CSA constructsa

FCR3 VAR2CSA constructAmino acid location in FCR3 VAR2CSA (accession no. AY372123 )End point titer of antiserum from rat:
123
Single domains
    DBL158-4388.1E+046.9E+051.3E+06
    DBL2545-8902.3E+052.3E+051.4E+05
    DBL31210-15876.4E+054.2E+043.3E+05
    DBL41583-19477.8E+043.3E+057.2E+04
    DBL51990-23284.6E+061.1E+062.6E+05
    DBL62307-2641>1E+07>1E+07>1E+07
    Interdomain 2929-12239.5E+044.6E+054.3E+04
Subdomains
    DBL1 Sub1+Sub258-2678.0E+069.7E+057.4E+05
    DBL1 Sub3256-4383.8E+043.7E+058.2E+04
    DBL2 Sub1+Sub2538-7541.6E+057.7E+054.1E+05
    DBL2 Sub3745-8931.2E+057.3E+046.8E+04
    DBL3 Sub1+Sub21210-14494.5E+053.2E+065.3E+05
    DBL3 Sub31444-15872.8E+044.8E+056.7E+05
    DBL4 Sub1+Sub21583-1789>1E+04>1E+04>1E+04
    DBL4 Sub2+Sub31638-1947>1E+04>1E+04>1E+04
    DBL4 Sub31784-1947>1E+04>1E+04>1E+04
Multidomains
    NTS+DBL1+DBL21-8932.3E+066.0E+061.8E+06
    DBL2+DBL3545-15875.0E+061.3E+063.1E+05
    DBL3+DBL41210-19474.8E+061.9E+065.8E+05
    DBL5+DBL61990-2641>1E+07>1E+071.6E+06
Chimeric domain DiCo DBL5Synthetic gene, GenBank accession no. FJ0288158.6E+054.2E+05Not done
Control domains
    FCR3 var1DBL3g1267-15805.1E+063.7E+059.1E+05
    3D7 F08_0141. DBL2b861-11937.1E+052.4E+061.8E+07
  • a The table describes the antigens cloned from FCR3 VAR2CSA used for immunizations. Antigen boundaries are indicated as start and stop amino acids from the coding sequence of VAR2CSA FCR3 (accession no. AY372123 ). Three rats were immunized per antigen, and the end point titer of the full bleed is indicated for each rat.