TABLE 1.

Genes targeted for mutagenesis

Locus tag (gene designation)aGene product (protein designation)No. of nucleotides/ residuesAmplified fragment(s) in nucleotidesPrimerSequence
Cj1478c (cadF)CadF960/320620cadF-FbTATTTCTATGGTTTAGCAGGTGGAG
cadF-RbGCTCTACCTTCTTTAGTGTCATTGC
Cj0628/Cj0629CapA3,436/1,1451321, 1635capA-FbTGAATCGAAGTGGAAAAATAGAAG
    (capA)capA-RbCCCATTTTTGTATCTTCATAACCT
capA-SstI-FcATGAGCTCAAAGTTGTTCCTAAGGGTAAAGC
capA-SstII-RcATACCGCGGAGTTTTATTCATAAATATTCCCTTTCC
capA-SstII-FcATACCGCGGGCTCAGTTTAATTATCTTTGGTAATC
capA-XhoI-RcATACTCGAGCATTTTACAAGCCCTATAAGAAGG
Cj0983 (jlpA)JlpA1,119/373868jlpA-FbTCTCAGGACTCTGGAATAAAGATTG
jlpA-RbGTGTGCTATAGTCACTAACAGGGATG
Cj0921c (peb1A)PEB1780/260560peb1A-FbTCTAGGTGCTTGTGTTGCATTTAG
peb1A-RbTGTCTACAGAAAACGCATCAACTC
Cj1279cCj1279c (FlpA)1,233/411832Cj1279c-FbTCAGAAGATGGCAAGGTTATAGAAG
Cj1279c-RbGTTATTGCTATTGATTCAGCTGGAC
Cj1349cCj1349c1,308/4361115Cj1349c-FbTATTTTTGATCTTACTCGTGCAATG
Cj1349c-RbTTAAGGTATAATCGACCCAATACGA
Cj1278cCj1278c1,179/3931033, 991Cj1278c-BamHI-FcATATAGGATCCGTATCGTTCTAGTGATGAAAATCC
Cj1278c-SstII-RcATATACCGCGGTTTTAAAATTTGGCACTACTGAGC
Cj1278c-SstII-FcATATACCGCGGGTTTAAAATATAATTTTTCTTGAAAATTAAGC
Cj1278c-BamHI-RcATATAGGATCCTTTTCAGAAACATCATTTTTCAAACG
  • a The gene number is from the genome sequence from C. jejuni NCTC 11168.

  • b Primer used to amplify a DNA fragment for the generation of a suicide vector (gene knockout) and/or the probe for dot blot hybridization.

  • c Primer used to amplify the DNA fragments for construction of the vectors to generate the mutants (i.e., capA and Cj1278c) via a double-crossover event. The two fragments were cloned into pBSK-Kan2 and disrupted by insertion of a Tetr cassette.