TABLE 5.

Immunization of mice with pools of proteins separated by preparative electrophoresis and protection against intraperitoneal challenge with N. meningitidis K454a

Challenge dose (CFU)VaccinebNo. of survivors (no. healthy)No. of survivors/ no. in group at 72 h
24 h52 h
2 × 107None5 (0)00/5
DE of whole cells5 (0)4 (2)4/5
<43-kDa pool5 (0)5 (5)5/5
43-65-kDa pool5 (0)4 (0)4/5
>67-kDa pool5 (0)1 (0)1/5
5 × 108No vaccine5 (0)00/5
DE of whole cells5 (0)1 (0)0/5
<43-kDa pool5 (0)3 (0)3/5
43-65-kDa pool5 (0)00/5
>67-kDa pool5 (0)00/5
  • a Vaccines were prepared in Freund's complete adjuvant (first immunization) or Freund's incomplete adjuvant (subsequent immunizations).0 activity plus two standard deviations (83 ELISA units).

  • b DE, detergent (Elugent) extract of N. lactamica whole cells.