TABLE 3.

Genes induced by iron and IdeRa

Rv no.GenemRNA ratioGene product
WT HI/LI inducedWT HI/ST22 HIWT HI/ST52 HI
0009ppiA2.7 ± 0.13.0 ± 0.31.0Peptidyl-prolyn-cis-trans isomerase
0338c2.8 ± 0.22.4 ± 0.41.0Iron-sulfur protein
1552bfrA2.1 ± 0.12.6 ± 0.21.0Bacterioferritin
3841bfrB11.5 ± 1.84.3 ± 0.73.8 ± 0.6Ferritin-like protein
  • a A DNA microarray was used to measure mRNA levels in cultures of M. tuberculosis H37Rv during exponential growth under iron-deficient (2 μM FeCl3) (LI) or -sufficient (50 μM FeCl3) (HI) conditions and in exponential cultures of strains ST22 and ST52 grown in iron-sufficient medium. Differences in gene-specific mRNA levels of twofold and higher are listed. Iron- and IdeR-induced genes were identified as genes induced under high-iron conditions in the wild-type strain (WT HI/LI > 2), not induced under high-iron conditions in the ideR mutant ST22 (WT HI/ST22 HI > 2), and induced under high-iron conditions in the complemented strain ST52 to levels comparable to those in the wild type under high-iron conditions (WT HI/ST52 HI = 1.0). The Genes are annotated as described by the Pasteur Institute on TUBERCULIST (http://genolist.pasteur.fr/Tuberculist).