TABLE 5.

IdeR-independent iron-induced genesa

Rv no.GenemRNA ratio (WT HI/LI)Gene productb
0706rplV1.6 ± 0.150S ribosomal protein L22
1252cIprE1.6 ± 0.1Lipoprotein
1305atpE1.6 ± 0.1ATP synthase c chain
1908cKatG1.7 ± 0.2Catalase-peroxidase
1943c1.7 ± 0.1CHP
25261.6 ± 0.1CHP
2549c1.6 ± 0.1Unknown
2550c2.0 ± 0.2Unknown
2741PE-PGRS2.0 ± 0.1PE-PGRS subfamily
2927c1.6 ± 0.1CHP
3075c1.7 ± 0.2CHP
3145nuoA1.7 ± 0.3NADH dehydrogenase chain A
3146nuoB1.8 ± 0.1NADH dehydrogenase chain B
3147nuoC1.6 ± 0.2NADH dehydrogenase chain C
3148nuoD1.7 ± 0.2NADH dehydrogenase chain D
3152nuoH1.8 ± 0.1NADH dehydrogenase chain H
3153nuoI1.6 ± 0.1NADH dehydrogenase chain I
3155nuoK1.6 ± 0.2NADH dehydrogenase chain K
3156nuoL1.7 ± 0.2NADH dehydrogenase chain L
3157nuoM1.6 ± 0.1NADH dehydrogenase chain M
3158nuoN1.6 ± 0.1NADH dehydrogenase chain N
3246mtrA2.0 ± 0.3Two-component response     regulator
33941.7 ± 0.2Unknown
  • a A DNA microarray was used to measure mRNA levels in cultures of M. tuberculosis H37Rv during exponential growth under iron-deficient (2 μM FeCl3) (LI) or -sufficient (50 μM FeCl3) (HI) conditions and in exponential cultures of strains ST22 and ST52 in iron-sufficient medium. Genes induced by 1.6-fold or more are listed. Iron-dependent, IdeR-independent genes were induced under high-iron conditions in the wild type (WT HI/LI > 1.6), and their expression under high-iron conditions was not affected in the ideR mutant. All these genes showed a WT HI/ST22 HI ratio of 1.0 (not shown). The genes are annotated as described by the Pasteur Institute on TUBERCULIST (http://genolist.pasteur.fr/Tuberculist). Genes that are separated by <50 bp and are probably coregulated are grouped.

  • b CHP, conserved hypothetical protein; CHMP, conserved hypothetical membrane protein.