TABLE 2.

DNA microarray analysis of gene expression changes in mouse hepatocytes stimulated with LPS: increased gene expressiona

FunctionFold changeGene product(s) and description
Cell death or apoptosis2.9ESTs, moderately similar to PI-3 catalytic subunit, beta isoform (Homo sapiens)
1.5Calpastatin
Liver metabolizing enzyme1.6Aldehyde dehydrogenase family 1, subfamily A4
1.6Selenophosphate synthetase 2
1.6Cytochrome P450, 2a5
1.6Alcohol dehydrogenase 5
1.6Spermine synthase
1.6Triosephosphate isomerase
1.6Uridine phosphorylase
1.5Phosphoglycerate kinase 1
1.5Mitochondrial acyl coenzyme A thioesterase
1.5Cytochrome P450, 2c29
1.5Cytochrome P450, 2a4
1.5Cytochrome P450, 2c37
1.5Paraoxonase 1
Signal transduction2.4Inhibitor of DNA binding 2
1.7RFG
1.6Mus musculus calcium/calmodulin-dependent protein kinase II delta mRNA, partial cds
1.5Protein tyrosine phosphatase, nonreceptor type 13 interacting protein
1.5Signal recognition particle, 9 kDa
1.5ESTs, highly similar to putative adenosine kinase (Saccharomyces cerevisiae)
1.5Protein kinase, interferon-inducible double-stranded RNA-dependent inhibitor
1.5Disabled homolog 1 (Drosophila)
1.5T-cell-specific GTPase
1.5Aplysia ras-related homolog N (RhoN)
1.5Bright and dead ringer gene product homologous protein Bdp
Stress response or antioxidant1.9Glutathione-S-transferase, alpha 3
1.8Glutathione-S-transferase, alpha 2 (Yc2)
1.6Glutathione-S-transferase, alpha 4
1.6Glutathione-S-transferase, mu 6
1.5Glutathione transferase, zeta 1 (maleylacetoacetate isomerase)
1.6Selenoprotein P, plasma, 1
Liver protein or acute-phase protein1.7Serum albumin variant
1.6Selenium binding protein 1
1.6Group-specific component
1.6Complement component 4 binding protein
1.6ESTs, highly similar to fibrinogen gamma-a and -b chain precursors (Rattus norvegicus)
1.6ESTs, highly similar to insulin-induced growth response protein cl-6 (Rattus norvegicus)
1.5Complement component factor I
1.5Apolipoprotein H
1.5Sterol carrier protein 2, liver
1.5Orosomucoid 1 (AGP)
DNA binding protein1.5Histone H2A.Z
Glucose response1.5Pleiotropic regulator 1, PRL1 homolog (Arabidopsis)
Transcription factor1.5ESTs, highly similar to hypothetical 18.5-kDa protein c12g12.05c (Schizosaccharomyces pombe)
1.5LIM only 2
Reverse transcriptase1.5ESTs, weakly similar to open reading frame (M. musculus)
Cell adhesion1.6Desmoglein 2
1.5P40-8, functional
Cysteine proteinase1.6Cathepsin L
Membrane protein1.5Flotillin 1
1.5Lymphocyte antigen 6 complex
Cell locomotion1.5Hyaluronan-mediated motility receptor (RHAMM)
Tight junction protein1.5Claudin 1
Protease inhibitor1.5Calpastatin
  • a Total RNA from cultured hepatocytes from C3H/HeN mice was subjected to DNA microarray analysis. The mRNA from normal and LPS (100-ng/ml)-treated (8 h) mice was labeled with cyanine-3-dUTP (green) and cyanine-5-dUTP (red), respectively. Positive fold change means gene expression was increased following treatment with LPS. The genes are sorted by function according to PubMed search. Only genes whose expression has been increased by at least 50% (1.5-fold) are shown here.