TABLE 4.

IdeR-independent, iron-repressed genesa

Rv no.GenemRNA ratio (WT LI/WT HI)Gene productb
0279cPE-PGRS2.2 ± 0.2PE-PGRS protein
0464c2.2 ± 0.2CHP
0465c4.3 ± 0.7Probable transcriptional regulator (PbsX/Xre family)
0467icl3.5 ± 0.4Isocitrate lyase
0676cmmpL52.8 ± 0.2Conserved large membrane protein
0677cmmpS53.2 ± 0.3Conserved small membrane protein
06922.7 ± 0.2CHP
0693pqqE2.5 ± 0.3Coenzyme PQQ synthesis protein E
0694lldD12.3 ± 0.2l-Lactate dehydrogenase (cytochrome)
1169c2.2 ± 0.2PE protein
1184c2.2 ± 0.1CHP
11952.2 ± 0.2PE protein
1393c2.4 ± 0.3FAD-containing monooxygenase
14614.7 ± 0.4CHP
14623.4 ± 0.6CHP
14633.3 ± 0.5Probable ABC transporter
14643.0 ± 0.3NifS-like protein
1465*2.6 ± 0.5NifU-like protein
14663.2 ± 0.6CHP
15202.1 ± 0.2Probable glycosyltransferase
2621c2.4 ± 0.3Probable transcriptional regulator
2794c2.7 ± 0.2CH; similar to proteins involved in vibriobactin and enterobactin synthesis
3229cdesA35.0 ± 2.7Acyl[ACP] desaturase
3230c2.5 ± 0.5Probable oxygenase
3614c2.1 ± 0.1CHP
3854cethA4.4 ± 0.4Probable monooxygenase
  • a A DNA microarray was used to measure mRNA levels in cultures of M. tuberculosis H37Rv during exponential growth under iron-deficient (2 μM FeCl3) (LI) or -sufficient (50 μM FeCl3) (HI) conditions and in exponential cultures of strains ST22 and ST52 in iron-sufficient medium. Differences in gene-specific RNA levels of twofold or more are listed. Genes contiguous to included genes and with a ratio of > 1.6 after the standard deviation was subtracted are also listed. Iron-dependent, IdeR-independent genes were derepressed under low-iron conditions in the wild-type (WT LI/HI > 2) and were not affected in their repression under high-iron conditions in the ideR mutant. All these genes showed a WT HI/ST22 HI ratio of 1.0 (not shown). The genes are annotated as described by the Pasteur Institute on TUBERCULIST (http://genolist.pasteur.fr/Tuberculist), except for that indicated by an asterisk. That gene is annotated based on an individual BLAST search. Genes that are separated by <50 bp and are probably coregulated are grouped.

  • b CHP, conserved hypothetical protein; CHMP, conserved hypothetical membrane protein.