IdeR-independent, iron-repressed genesa

Rv no.GenemRNA ratio (WT LI/WT HI)Gene productb
0279cPE-PGRS2.2 ± 0.2PE-PGRS protein
0464c2.2 ± 0.2CHP
0465c4.3 ± 0.7Probable transcriptional regulator (PbsX/Xre family)
0467icl3.5 ± 0.4Isocitrate lyase
0676cmmpL52.8 ± 0.2Conserved large membrane protein
0677cmmpS53.2 ± 0.3Conserved small membrane protein
06922.7 ± 0.2CHP
0693pqqE2.5 ± 0.3Coenzyme PQQ synthesis protein E
0694lldD12.3 ± 0.2l-Lactate dehydrogenase (cytochrome)
1169c2.2 ± 0.2PE protein
1184c2.2 ± 0.1CHP
11952.2 ± 0.2PE protein
1393c2.4 ± 0.3FAD-containing monooxygenase
14614.7 ± 0.4CHP
14623.4 ± 0.6CHP
14633.3 ± 0.5Probable ABC transporter
14643.0 ± 0.3NifS-like protein
1465*2.6 ± 0.5NifU-like protein
14663.2 ± 0.6CHP
15202.1 ± 0.2Probable glycosyltransferase
2621c2.4 ± 0.3Probable transcriptional regulator
2794c2.7 ± 0.2CH; similar to proteins involved in vibriobactin and enterobactin synthesis
3229cdesA35.0 ± 2.7Acyl[ACP] desaturase
3230c2.5 ± 0.5Probable oxygenase
3614c2.1 ± 0.1CHP
3854cethA4.4 ± 0.4Probable monooxygenase
  • a A DNA microarray was used to measure mRNA levels in cultures of M. tuberculosis H37Rv during exponential growth under iron-deficient (2 μM FeCl3) (LI) or -sufficient (50 μM FeCl3) (HI) conditions and in exponential cultures of strains ST22 and ST52 in iron-sufficient medium. Differences in gene-specific RNA levels of twofold or more are listed. Genes contiguous to included genes and with a ratio of > 1.6 after the standard deviation was subtracted are also listed. Iron-dependent, IdeR-independent genes were derepressed under low-iron conditions in the wild-type (WT LI/HI > 2) and were not affected in their repression under high-iron conditions in the ideR mutant. All these genes showed a WT HI/ST22 HI ratio of 1.0 (not shown). The genes are annotated as described by the Pasteur Institute on TUBERCULIST (, except for that indicated by an asterisk. That gene is annotated based on an individual BLAST search. Genes that are separated by <50 bp and are probably coregulated are grouped.

  • b CHP, conserved hypothetical protein; CHMP, conserved hypothetical membrane protein.