TABLE 2.

TTSS-deficient noncytotoxic mutants

MutantGene(s)GenBank accession no.Cytotoxicity (% of CHA)aNo. of gene occurenceTTSS functionb
12exsDPA171431
21phoAPA329631
22pscLPA172531
23rpoNPA446221
26mreCPA448081
43ppxPA5241101
36, 37pcrVPA170682−(PopB/D)
27, 32, 46dsbAPA548923
24, 25, 44, 52aceAPA501514Small amount
9, 11, 13, 39, 42, 45, 47, 50, 53, 54aceBPA5016310Small amount
44 (pDD2)aceA, with constitutive exsA80+
9 (pDD2)aceB, with constitutive exsA80+
9 (pAG710)aceB, complemented aceB70+
  • a For further details, see the text. Values are means of three experiments.

  • b +, TTSS function is present; −, no TTSS function.