TABLE 1.

Quantification of intracellular proliferation by UPEC

Parameter% of PD07i cellsa:
NU14NU14-1b
ControlFilipinControlFilipin
No. of bacteria/cell
    1-578.5 ± 3.282.4 ± 0.9100 ± 0.097.3 ± 1.2
    6-2017.9 ± 3.8†9.7 ± 1.6†0 ± 0.00.7 ± 1.2
    >203.6 ± 0.92.6 ± 0.60 ± 0.00 ± 0.0
IBCs0 ± 0.05.2 ± 1.2**0 ± 0.02.0 ± 0.02**
Total infected cells19.8 ± 3.516.4 ± 1.01.2 ± 0.2*2.1 ± 0.2*
  • a UPEC intracellular proliferation was determined by surveying 50 to 100 PD07i cells containing intracellular bacteria and quantifying the number of cells containing 1 to 5 bacteria, 6 to 20 bacteria, >20 bacteria, or IBCs after vehicle control or filipin pretreatment. *, P < 0.05; **, P < 0.05; †, P < 0.05.

  • b NU14-1 is an isogenic fimH mutant.